IJCRR - 6(3), February, 2014
VALIDATION OF NEWLY FORMULATED LAPORTEA ARISHTA BY USING DIFFERENT ANALYTICAL METHODS
Author: Deepa P., Seena
Traditional medicines have nurtured the knowledge of natural remedies against diseases since ages. Growing awareness about harmful side effects of modern medicine has led to interest in ayurveda. Laportea interrupta (L) Chew of family Urticaceae having common name Hawaii woodnettle is an herb having many traditional uses. As a preliminary step physicochemical analysis of crude drug, Laportea interrupta-leaves were performed. Physicochemical analysis includes Rodent Contamination, Foreign Organic Matter, Insect Infestation Total Ash Value, Acid Insoluble Ash, Water Insoluble Ash, Sulphated Ash and Moisture Content. Laportea interrupta leaves extracted in soxhlet apparatus using ethyl acetate, petroleum ether, chloroform, methanol and water for identification of constituents by qualitative phytochemical analysis (tests for protein, carbohydrates, phenols and tannins, flavanoids, saponins, glycosides, steroids, terpenoids and alkaloids) and quantitative phytochemical analysis (total phenol content and total flavanoid content). The collected leaves of Laportea interrupta was used to prepare arishta. After preparing the arishta the organoleptic characteristics (colour, odour, taste and appearance), the physicochemical analysis (pH, acid value, alcohol content, total solid content, viscosity and refractive index), quantitative analysis (alcohol content by spectrophotometry by dichromate method, total reducing sugar, total phenol content and total flavanoid content), anti-oxidant activity determination ( Ferric Thiocyanate method, Thiobarbituric acid method and Total Antioxidant Activity by FRAP method) were performed. LCMS was performed for determining various constituents (Qualitative analysis) and standardisation of formulation was done by using UV and HPLC. The above formulated Laportea Arishta was validated by using different analytical equipment’sas per ICH guidelines.
Keywords: Arishta, Physicochemical analysis, Organoleptic characteristics
Deepa P., Seena. VALIDATION OF NEWLY FORMULATED LAPORTEA ARISHTA BY USING DIFFERENT ANALYTICAL METHODS International Journal of Current Research and Review. 6(3), February, 22-34
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