IJCRR - 6(7), April, 2014
Pages: 01-10
OPTIMUM DIETARY INCLUSION LEVEL OF BACILLUS COAGULANS FOR GROWTH AND DIGESTIBILITY IMPROVEMENT FOR CATLA CATLA (HAMILTON)
Author: Anita Bhatnagar, Shashi Raparia
Category: General Sciences
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Abstract:
Purpose: The present studies were conducted to evaluate the optimum inclusion levels of probiotic Bacillus coagulans, isolated from the intestine of Catla catla for improved growth performance and nutrient retention. Methods: Catla fingerlings (avg. wt. 0.2±0.02 g) were fed on isocaloric and isonitrogenous diets supplemented with 2×104 (D1), 2×105(D2) and 2×106(D3) Bacillus coagulans cells 100g-1 of feed for 90 days @ 4 % body weight per day in two equal installments in three replicates. The control treatment (DC) was not supplemented with Bacillus coagulans. Growth and digestibility parameters and intestinal enzyme activities were monitored. Results: The growth of fish in terms of live weight gain (g), growth per day in percentage body weight and specific growth rate were significantly (P<0.05) high in feed D2 in comparison to other dietary treatments. Also, significantly (P<0.05) high values of Apparent protein digestibility (APD), Gross conversion efficiency (GCE) and Protein efficiency ratio (PER) lower FCR (1.64±0.02) was observed in the dietary treatment D2. Significantly (P?0.05) high values of digestive enzyme activities (protease, amylase and cellulose), carcass protein, and low excretion of metabolites (ammonia and phosphates) were also observed in feed D2. Conclusion: Results indicated that that supplementation of 2×105 cells 100g-1of probiotics, B. coagulans could be used to enhance growth performance and nutrient retention of C. catla.
Keywords: Bacillus coagulans, Catla catla, Dietary probiotic, Growth performance, Intestinal enzymes
Citation:
Anita Bhatnagar, Shashi Raparia. OPTIMUM DIETARY INCLUSION LEVEL OF BACILLUS COAGULANS FOR GROWTH AND DIGESTIBILITY IMPROVEMENT FOR CATLA CATLA (HAMILTON) International Journal of Current Research and Review. 6(7), April, 01-10
References:
REFERENCES
1. Austin B, Austin DA. Bacterial Fish Pathogen: Disease of Farmed and Wild Fish. 3rd ed. (Revised). Goldalming: Springer-Praxix; 1999.
2. Alderman DJ, Hasting TS. Antibiotic use in aquaculture: development of antibiotic resistance potential for consumer health risk. Int J Food Sci Technol 1998; 33:139-55.
3. Bisht A, Singh UP, Pandey NN. Bacillus subtilis as potential probiotic for enhancing growth in fingerlings of common carp (Cyprinuscarpio L.). Indian J Fish 2012; 59(3):103-8.
4. Guarner F, Malagelada JR. Gut flora in health and disease. The Lacent 2003; 360:512-9.
5. Moriarty DJW. Microbial Biotechnology: a key ingredient for sustainable aquaculture, Infofish Int 1996; 4:29-33.
6. Vine NG, Leukes WD, Kaiser H, Daya S, Baxter J, Hecht T. Competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus. J Fish Dis 2004; 27(6):319-26.
7. Ziaei-Nejad S. The effect of Bacillus sp. bacteria as a probiotic on growth, survival and digestive enzyme activity of Indian white Shrimp, Fenneropenaeusindicus, larvae and post larvae [Msc thesis]. Tehran University; 2004.
8. Moriarty DJW. Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture 1998; 164:351-8.
9. Ringpipat S, Phianphak W, Piyatiratitivorakul S. Effects of probiotic bacterium on black tiger shrimp Penaeusmonodon survival and growth. Aquaculture 1998; 167:301-13.
10. Zhou X, Tian Z, Wang Y, Li W. Effect of treatment with probiotics as water additives on tilapia (Oreochromisniloticus) growth performance and immune response. Fish Physiol Biochem. (doi 10.1007/s10695-009- 9320-z).
11. Bhatnagar A, Raparia S, Kumari S. Influence of isolated Bacillus coagulans on growth performance and digestive enzyme activities of Catla catla. JNNST 2012; 6(3):225-35.
12. APHA. Standard methods for the examination of water and waste water. 20th ed. American Public Health Association. New York; 1998.
13. Sumagaysay-Chavoso NS. Nitrogen and phosphorus digestibility and excretion of different size groups of milkfish (ChanoschanosForsskal) for formulated and natural food –based diets. Aquac Res 2003; 34(5):407–18.
14. Walter HE. Probionases : Methods with haemoglobin, casein and azocoll as substrates. In : Bergmeyer HU, editor. Methods of Enzymatic Analysis, Vol. V. Verlag Chemic: Weinheim; 1984. p. 270-7.
15. Sawhney SK, Singh R. Introductory Practical Biochemistry. Narosa Publishing House; 2000. p.452.
16. Sadasivam S, A Manickam. Biochemical methods. New Delhi, India: New Age International Publishers; 1996.
17. AOAC, (Association of Official Analytical Chemists). Official methods of analysis. Association of Official Analytical Chemists Incorporation, Arlington, USA; 1995. p.684.
18. Furukawa A, Tuskahara H. On the acid digestion method for determination Chromic oxide as an indicator substance in the study of digestibility in fish. Bull jap soc sci fish 1966; 32:502–6.
19. Steffens W. Principles of fish nutrition.Chichester, NY: Horwood; 1989.
20. Cho CY, Slinger S J, Bayley H S. Bioenergetics of salmonids fishes: energy intake, expenditure and productivity. Comp Biochem Physiol B 1982; 73: 25–41.
21. Henken AM, H Lucas, PAT, Tijseen MAM Michiels. A comparison between methods used to determine the energy content of feed, fish and faecal samples. Aquaculture 1986; 58:195–201.
22. Brett JR, Groves TTD. Physiological energetics. In: Hoar WS, Randall DJ, Brett JR editors. Fish Physiol vol. 8. New York: Academic Press; 1979. p.280–352.
23. Duncan DB. Multiple range and multiple Ftests. Biometrics 1955; 11: 1-42.
24. Bazaz MM, Keshavanath P. Effect of feeding levels of sardine oil on growth, muscle composition and digestive enzyme activities of Masheer Tor khudree. Aquaculture 1993; 115: 111-9.
25. Ghosh K, Sen SK, Ray AK. Supplementation of an isolated fish gut bacterium, Bacillus circulans, in formulated diets for rohu, Labeo rohita fingerlings. Bamidgeh 2003; 55 (1): 13- 21.
26. Wada H, Marino Y. Comparative studies on glutamic-oxaloacetic transaminases from the mitochondrial and soluble fractions of mammalian tissues. In: Harris RS, Wool IG, Loraine JA, editors. Vitamins and Hormones. New York: Academic Press; 1964. p.411-44.
27. Phillips AM. Nutrition, digestion and energy utilization. In: W.S. Hoar W S, Randall D J, editors. Fish Physiology. vol 1. New York: Academic Pres; 1969. p.391-432.
28. Dong H, Fredrickson JK, Kennedy DW, Zachara JM, Kukkadapu RK, Onstott TC. Mineral Transformation associated with the microbial reduction of magnetite. Chem Geol 2000; 169: 299-318.
29. Swain SK, Rangacharyulu PV, Sarakar S, Das KM. Effect of a probiotic supplement on growth, nutrient utilization and carcass composition in Mrigal fry. J Aquac 1996; 4: 29-35.
30. Mohanty SN, Swain SK, Tripathi SD. Rearing of (Catla catla Ham) spawn on formulated diets. J Aquac Trop 1996; 11:253-258.
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