IJCRR - Vol 09 Issue 09, May, 2017
Elucidation of the role of the minC gene in filament formation by Listeria monocytogenes under stress conditions
Author: Satyajit B. Kale, Swapnil P. Doijad, Krupali V. Poharkar, Sandeep Garg, Ajay D. Pathak, Abhay V. Raorane, Deepak B. Rawool, Nitin V. Kurkure, Sukhadeo B. Barbuddhe
Aim: The study was conducted to understand structural changes in cell morphology of stress tolerant Listeria monocytogenes strains after exposure to the different food related stresses and to investigate the involvement of the minC gene in filament formation under stress as a putative mechanism. Methods: Morphological changes in L. monocytogenes were studied under the stresses of high salt concentration (12.5%), extreme pH (4.5 and 9.0) and low temperature (4°C). The structural changes were recorded employing light and electron microscopy. The expression of the minC gene under stress was studied by qPCR. Results: Long filament formations were observed under salt stress, while, no structural changes could be observed for isolates grown in extreme pH and low temperature stresses. Scanning electron microscopic studies showed 3-10 times elongation of cells under stress which got reverted to normal size after removal of stress. Interestingly, it was noted that with the increase in stress, rod shaped cells became elongated. Six to 11 fold expression of the minC gene was observed under high salt stress. Conclusion: The results suggested that the filament formation could be one of the mechanisms by bacteria to tolerate high salt stress. It also supported the hypothesis that the minC gene over-expression could be the factor behind filamentous morphology
Keywords: Listeria mnocytogenes, Serogroups, Tolerance, Salt, minC, Morphology
Satyajit B. Kale, Swapnil P. Doijad, Krupali V. Poharkar, Sandeep Garg, Ajay D. Pathak, Abhay V. Raorane, Deepak B. Rawool, Nitin V. Kurkure, Sukhadeo B. Barbuddhe. Elucidation of the role of the minC gene in filament formation by Listeria monocytogenes under stress conditions International Journal of Current Research and Review. Vol 09 Issue 09, May, 09-13
1. Bereksi N, Gavini F, Bénézech T, Faille C.. Growth, morphology and surface properties of Listeria monocytogenes Scott A and LO28 under saline and acid environments. J Appl Microbiol 2002; 92: 556–565.
2. Buchanan R, Lindqvist R, Ross T, Smith M, Todd E, Whiting R.. Risk assessment of Listeria monocytogenes in ready-to-eat foods. Microbiological Risk Assessment Series, 4. Food and Agriculture Organization of the United Nations, 2004.
3. Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 2004; 42: 3819-3822.
4. Farber JM, Coates F, Daley E. Minimum water activity requirements of the growth of Listeria monocytogenes. Lett Appl Microbiol 1992;15:103-105.
5. Gandhi M & Chikindas M L.. Listeria: a foodborne pathogen that knows how to survive. Int J Food Microbiol 2007;113:1–15.
6. Gianfranceschi M, Gattuso A, Tartaro S, Aureli P. Incidence of Listeria monocytogenes in food and environmental samples in Italy between 1990 and 1999: Serotype distribution in food, environmental and clinical samples. Eur J Epidemiol 2002;18: 1001–1006.
7. Giotis E S, Blair IS, McDowell DA. Morphological changes in Listeria monocytogenes subjected to sublethal alkaline stress. Int J food Microbol 2007;120:250–258.
8. Isom L L, Khambatta ZS, Molus J L, Akers DF, Martin S E. Filament formation in Listeria monocytogenes, J Food Prot 1995; 58:1031–1033.
9. Jones T, Gill C O, McMullen L, The behavior of log phase Escherichia coli at temperatures below the minimum for sustained growth. Food Microbiology 2002; 19:83–90.
10. Jones TH, Vail KM, McMullen LM. Filament formation by foodborne bacteria under sublethal stress. Inter J Food Microbiol 2013;165: 97–110.
11. Liu D, Lawrence ML, Ainsworth A J, Austin FW. Comparative assessment of acid, alkali and salt tolerance in Listeria monocytogenes virulent and avirulent strains. FEMS Microbiol Lett 2005;243: 373-378.
12. Magalhaes R, Ferreira V, Brandão T R, Palencia RC, Almeida G, Teixeira P. Persistent and non-persistent strains of Listeria monocytogenes: A focus on growth kinetics under different temperature, salt, and pH conditions and their sensitivity to sanitizers, Food Microbiol 2016;57:103-108.
13. Pratt AL, Chen B, Czuprynski CJ, Wong ACL, Kaspar CW. Characterization of osmotically induced filaments of Salmonella enterica. App Enviro Microbiol 2012;78:6704–6713.
14. Rothfield L, Taghbalout A and Shih, Y.L., Spatial control of bacterial division-site placement. Nature Rev Microbiol 2005; 3:959–968.
15. Sartor C, Grégoire E, Albanèse J, Fournier PE. Invasive Listeria monocytogenes infection after liver transplantation: a lifethreatening condition, Lancet. 2015;6736: 61831-61836.
16. Scheffers D -J. The effect of MinC on FtsZ polymerization is pH dependent and can be counteracted by ZapA. FEBS Letters 2008;582: 2601–2608.
17. Shabala L, Lee SH, Cannesson P, Ross T.. Acid and NaCl limits to growth of Listeria monocytogenes and influence of sequence of inimical acid and NaCl levels on inactivation kinetics, J Food Prot 2008;71:1169-1177.
18. Vail KM, McMullen LM, Jones TH. Growth and filamentation of cold-adapted, log phase Listeria monocytogenes exposed to salt, acid, or alkali stress at 3°C. J Food Prot 2012; 75:2142– 2150.