IJCRR - 6(15), August, 2014
Pages: 45-48
IN-VITRO LEUCOAGGLUTINATION: A STUDY OF 11 CASES
Author: Kavita Gupta, Deepak Nayak M., Chethan Manohar, Sushma V. Belurkar
Category:
[Download PDF]
Abstract:
Objective: Cold agglutination of red blood cells and platelets are well recognized and extensively studied in literature. However aggregation of leukocytes in vitro is rare and may result in spuriously low leucocyte count. We present a case series of 11 patients with spurious leukopenia due to leucoagglutination in-vitro. Methods: A retrospective study was conducted at Clinical laboratory and Haematology division, Kasturba Hospital, compiling 11 cases which showed WBC clumping on the peripheral smear in the last 2 years. Results: The aetiologies ranged from immune mediated (4 cases), liver diseases (2 cases) and infections/drug induced (5 cases). The incubation of samples at 37° C for 30 minutes caused disaggregation of leucocytes; thus denoting the role of temperature dependent antibody.Conclusion: Leucoagglutination in-vitro can be linked to an immunological phenomenon, possibly due to a temperature-dependent
antibody
Keywords: Leucoaggregates, EDTA (Ethylenediaminetetraacetic acid), antibody, leucocytes
Citation:
Kavita Gupta, Deepak Nayak M., Chethan Manohar, Sushma V. Belurkar. IN-VITRO LEUCOAGGLUTINATION: A STUDY OF 11 CASES International Journal of Current Research and Review. 6(15), August, 45-48
References:
1. Hillyer CD, Knopf AN, Berkman EM. EDTA-dependent leucoagglutination. Am J Clin Pathol 1990; 94:458–461.
2. Lesesve JF, Haristoy X, Thouvenin M, Latger-Cannard V, Buisine J, Lecompte T. Pseudoleukopenia due to in vitro leukocyte agglutination polynuclear neutrophils: experience of a laboratory, review of the literature and future management. Ann Biol Clin (Paris)2000; 58:417–424.
3. Savage RA. Analytic inaccuracy resulting from hematology specimen characteristics. Three cases of clinically misleading artefacts affecting white blood cell and platelet counts. Am J Clin Pathol 1989;92:295–299.
4. Epstein HD, Kruskall MS. Spurious leukopenia due to in vitro granulocyte aggregation. Am J Clin Pathol 1988;89:652–655.
5. Guibaud S, Plumet-Leger A, Frobert Y. Transient neutrophil aggregation in a patient with infectious mononucleosis. Am J Clin Pathol 1983; 80:883–884.
6. Moraglio D, Banfi G, Arnelli A. Association of pseudothrombocytopenia and pseudoleukopenia: Evidence for different pathogenic mechanisms. Scand J Clin Lab Invest 1994;54:257–265.
7. Lombarts, A. J. P. F., de Kieviet W. Recognition and prevention of pseudothrombocytopenia and concomitant pseudoleukocytosis. Am J Clin Pathol 1988;89:634-639.
8. Claviez A, Horst HA, Santer R, Suttorp M. Neutrophil aggregates in a 13-year-old girl: a rare hematological phenomenon. Ann Hematol 2003; 82:251–253.
9. Glasser L. Pseudo-neutropenia secondary to leukoagglutination. Am J Hematol 2005;80:147.
10. Carr ME, Whitehead J, Carlson P, et al. Case report: Immunoglobulin M mediated, temperature-dependent neutrophil agglutination as a cause of pseudoneutropenia. Am J Med Sci. 1996;311:92–95.
11. Lesesve JF, Haristoy X, Lecompte T. EDTA-dependent leucoagglutination. Clin Lab Hematol 2002;24:67–69.
12. Savage, R. A. (1984) Pseudoleukocytosis due to EDTA induced platelet clumping. Am J Clin Pathol 1984; 81:317-322.
13. Beckman Coulter. Coulter LH750 system: Operation principles. Operator’s Guide. 2004:9.
14. Bagdasaryan R, Zhou Z, Tierno B, et al. Neutrophil VCS parameters are superior indicators for acute infection. Lab Hematol 2007;13:12–16.
|