IJCRR - Vol 06 Issue 17, September, 2014
PREVALENCE AND ANTIBIOGRAM OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES AT A TERTIARY CARE HOSPITAL IN BANGALORE, SOUTH INDIA
Author: Kala Yadhav M. L., Gayathri J. Panicker
Background: The emergence of Methicillin-resistant Staphylococcus aureus (MRSA) has posed a serious therapeutic challenge. MRSA is an important cause of nosocomial infections worldwide. Objectives: The aim of this study was to determine the prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) from clinical samples in a tertiary care hospital and to analyse the antibiotic susceptibility patterns of MRSA isolates. Materials and Methods: Various clinical specimens were cultured and staphylococcus aureus was identified using standard tests like catalase test, slide and tube coagulase and growth on Mannitol salt Agar. All staph isolates were then tested for routine antibiotic sensitivity by Kirby - Bauer disc – diffusion method following CLSI guidelines. MRSA were then identified from amongst the Staph isolates by using cefoxitin (10 mcg) discs by the disc-diffusion method. The D-test was performed on all isolates of Staphylococcus aureus to identify erythromycin induced clindamycin resistance. Results: The prevalence of MRSA in our study was found to be 66.84%. Linezolid and Vancomycin proved to be effective against 93.89% and 89.31% of MRSA isolates. There was statistically significant resistance to Penicillin,Cefoxitin and Oxacillin (p<0.0001) and significant sensistivity to Linezolid and Vancomycin among MRSA. Also, 35.11% of MRSA isolates and 24.62% of MSSA isolates showed erythromycin induced clindamycin resistance in the study. Conclusion: MRSA infection in the hospital set up is on the rise and is a cause of worry due to resistance to commonly prescribed drugs. Regular surveillance and antibiotic sensitivity testing in hospitals can help to formulate antibiotic and other strategies for reducing the load and severity of MRSA infections world-over.
Keywords: Staphylococcus aureus, MRSA, Cefoxitin, Clindamycin, Linezolid, Vancomycin.
Kala Yadhav M. L., Gayathri J. Panicker. PREVALENCE AND ANTIBIOGRAM OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES AT A TERTIARY CARE HOSPITAL IN BANGALORE, SOUTH INDIA International Journal of Current Research and Review. Vol 06 Issue 17, September, 37-40
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