IJCRR - 6(18), September, 2014
Pages: 24-28
COMPARISON OF MICROBIAL FLORA BY DEEP TISSUE BIOPSY AND SUPERFICIAL SWAB CULTURE OF SPECIMEN COLLECTED FROM VARIOUS ANATOMICAL SITES IN EARLY WOUND INFECTIONS
Author: Saleem M., Joseph Pushpa Innocent D.
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Abstract:
The aim of this study is to evaluate and to compare the microorganisms isolated by two different methods of specimens collected from different anatomical sites in early wound infection, further to study the type of organisms grown in those sites. A total of 62 specimens were collected from 32 patients treated in Karpaga Vinayaga Institute of Medical sciences and Research Centre , during the period of six months from 15 .09 .13 to 15.03.14.Specimens were collected from gluteal, thigh, knee, leg, foot, breast, umbilical and genital regions. Initially a specimen was collected aseptically by common swab method from the wound and then the wound was cleaned, deep tissue biopsy was taken by scraping from the base of the same site of the wound to compare the two different methods of collection of specimens. Specimens were cultured by standard microbiological methods and the isolates were identified by standard biochemical methods. Among the 32 cases 7 were culture negative and the remaining 25 were culture positive. Most of the wound infections were presented in the foot 59.3% followed by leg 9.3 %. Knee, umbilical, gluteal and breast were presented with 6.2% of infection rate. Thigh and genital regions showed only 3.1% each. The commonest isolate was Staphylococcus aureus 41.2%. Out of the 14 isolates 12 were isolated from swab culture (35.3%). The next predominant organism isolated was E. coli which was 20.6 %, out of which 11.8% were isolated from deep tissue culture. Other isolates were Klebsiella 11.8%, Proteus and Pseudomonas spp were 8.8% each, Streptococcus 5.9% and Acinetobactor 2.9%. On comparison with the rate of isolation of organisms, it was more in swab culture (73.5%) than in the deep tissue culture (26.5%). Staphylococcus aureus found to be the most common Gram positive organism in the swab culture and E.coli was the more common Gram negative organism isolated in deep tissue culture. Out of 25 culture positive cases, 2 of them showed the same organisms both in swab culture and in deep tissue culture (8%). However the organisms isolated in swab culture were highly differ with deep tissue culture (28%). This study results reveal that the wound infections commonly seen in foot followed by leg. The common organism seen in swab culture is Staphylococcus aureus and the common Gram negative organism isolated was E.coli which is more predominate in deep tissue culture.
Keywords: Wound infections, Deep tissue culture, Superficial swab culture
Citation:
Saleem M., Joseph Pushpa Innocent D.. COMPARISON OF MICROBIAL FLORA BY DEEP TISSUE BIOPSY AND SUPERFICIAL SWAB CULTURE OF SPECIMEN COLLECTED FROM VARIOUS ANATOMICAL SITES IN EARLY WOUND INFECTIONS International Journal of Current Research and Review. 6(18), September, 24-28
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