IJCRR - 7(14), July, 2015
IL-3 AND GM-CSF INDUCES INCREASED RECRUITMENT OF LYMPHOCYTES IN PERIPHERAL BLOOD IN ENU INDUCED LEUKEMIA MOUSE MODEL
Author: Ashish Kumar Singha, Bhaskar Bhattacharjee, Debasish Maiti
Aim: The enrolment of blood progenitor cells from the bone marrow into peripheral blood or mobilization is required in leukemia following treatment with chemotherapy and/or cytokines. In this experiment, we examined the mobilization of lymphocytes from bone marrow to peripheral blood using interleukin-3 (IL-3) and granulocyte monocyte-colony stimulating factor (GM-CSF) in experimentally induced leukemia model.
Methods: In our Laboratory, leukemia is induced in Balb/C mice by challenging with N’-N’ Ethylnitrosourea (ENU) at the dose of 80mg/kg body twice in one week interval and confirmed by staining of peripheral blood and bone marrow smear. After confirmation of leukemia induction, animals were received rmIL-3 and rmGM-CSF in combination for consecutive four days. Leishman’s staining of peripheral blood smear and isolation of lymphocytes by percoll gradient method used in this study to determine the cell count.
Results: Total leukocyte count exhibits 6 folds higher in number in leukemia group compared to control group. After combination of treatment the total leukocyte count was same as leukemic control group until the sacrifice of animals. But the lymphocyte count increased around 2.6 fold after combination of IL-3 and GM-CSF treatment to leukemic mice.
Conclusion: This data suggests the mobilization of lymphocytes from bone marrow to peripheral blood may be immunologically beneficial to combat against tumor cells
Keywords: Mobilization, Leukemia, ENU, interleukin-3, Granulocyte monocyte colony, Stimulating factor
Ashish Kumar Singha, Bhaskar Bhattacharjee, Debasish Maiti. IL-3 AND GM-CSF INDUCES INCREASED RECRUITMENT OF LYMPHOCYTES IN PERIPHERAL BLOOD IN ENU INDUCED LEUKEMIA MOUSE MODEL International Journal of Current Research and Review. 7(14), July, 49-53
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