IJCRR - Vol 07 Issue 01, January, 2015
EFFECT OF DIFFERENT PARAMETERS ON BIODECOLOURIZATION OF AZO REACTIVE RED RB DYE FROM TEXTILE EFFLUENT
Author: T. Madhuri, B. Lakshmi Kalyani, P. Suvarnalatha Devi
Category: General Sciences
The aim of the present study is to isolate textile dye effluent degrading organisms. The synthetic textile dye effluents released into the environment, pollute soil and water ecosystem. Many of these effluents are toxic, carcinogenic and mutagenic in nature which effect aquatic and soil flora and fauna. Thus there is a need of processing these effluents before releasing into the environment. The Bacteria was isolated from soil contaminated with textile dye effluent by serial dilution followed by pour plating technique on nutrient agar medium. The ability of degradation was assessed by decolourization assay. Four isolates (RR1, RR2, RR3 and RR4) had the ability to degrade the dye effluent at different concentrations. The effect of pH, temperature, carbon and nitrogen sources and time course of decolourization was observed. The isolated RR2 and RR3 showed significant decolourization of dye at 600ppm.The ideal temperature was 370C and pH 7 and 9. Both isolates RR2 and RR3 showed optimum growth in media supplemented with sucrose and glucose as carbon source and RR2 showed good growth in ammonium sulphate and RR3 in peptone as a nitrogen source. The result concludes that the RR2 and RR3 isolates showed marked decolourization for textile dye effluent.
Keywords: Azo dye, Decolourization, Bioremediation, Red RB
T. Madhuri, B. Lakshmi Kalyani, P. Suvarnalatha Devi. EFFECT OF DIFFERENT PARAMETERS ON BIODECOLOURIZATION OF AZO REACTIVE RED RB DYE FROM TEXTILE EFFLUENT International Journal of Current Research and Review. Vol 07 Issue 01, January, 13-19
Ajay kumar Pandey and Vinay Dubey. Biodegradation of Azo dye reactive red BL by Alcaligenes sp. AA09. International Journal of Engineering and Science 2012; 1: 54-60.
2. Aksu Z, Kilic N, Ertugrul V and Donmez G. Inhibitory effects of chromium (Vl) and Remazol black onchromium (Vl) and dye stuff removals by Trametesversicolor. Enzyme and Microbial Technology 2007;40: 1167-1174.
3. Carliell C M, Barclay S J, Shaw C, Wheatley A D and Buckley C A. The effect of salts used in textile dyeing on microbial decolourization of a reactive azo dye. Environmental Technology 1998; 19(11): 1133–1137.
4. Cetin D and DonmezG.“Decolourization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic conditions” Enzymes and Microbial Technology 2006; 38: 926-930.
5. Chen K C, Hung W T, Wu J Y and Houng J Y. Microbial decolourization of azo dyes by Proteus mirabilis. Journal of Industrial Microbiology and Biotechnology 1999; 23: 686- 690.
6. Chen K C, Jane Y W, Liou D J and Sz-Chwun J Decolourization textile dyes by newly isolated bacterial strain. Journal of Biotechnology 2003; 101: 57-68.
7. Dawkar V V, Jadhav U U, Jadhav S U and Govindwar S P. Biodegradation of disperse textile dye Brown 3REL by newly isolated Bacillus sp. VUS. Journal of Applied Microbiology 2008; 105: 14-24.
8. Dykes G A, Timm R G and Von Holy A. Azoreductase activity in bacteria associated with the greening of instant chocolate puddings. Applied Environmental Microbiology 1994; 60: 3027-3029.
9. EI-Sersy N A. bioremediation of methylene blue by Bacillus thuringiensis 4G 1: application of statistical designs and surface plots for optimization. Biotechnology 2007; 6 (1): 34-39.
10. Feng J, Cerniglia C E and Chen H. Toxicological significance of azo dye metabolism by human intestinal microbiota. Front Biosciences (Elite Ed) 2012; 4: 568-586.
11. Forgacs E, Cserhati T and Oros G. Removal of synthetic dyes from wastewaters: Review on Environmental International 2004; 30: 953–971.
12. Gupta R, Gigras P, Mohapatra H, Goswami V K and Chauhan B. Microbial α-amylases: a biotechnological perspective. Process Biochemistry2003; 38:1599- 1616.
13. Hao O J, Kim H and Chaing P C. Decolourization of wastewater critical reviews. Environmental and Science Technology 2000; 30:449-505.
14. Jacob Thomson. Impact of Industries on the Ground Water Quality of Tiruppur and its Ethical implications, Ph.D. Thesis, Dept. of Zoology, University of Madras, Chennai 1998.
15. Jadhav S U, Kalme S D and Govindwar S P. Biodegradation of methyl red by Galactomyces geotrichum MTCC 1360. Intenational Biodeterioration and Biodegradation 2008; 62: 135-142.
16. Joshni T Chacko and Subramaniam K. Enzymatic degradation of Azo dyes- A Review. International Journal of Environmental Sciences 2011; 1:6.
17. Jothimani P and Prabhakaran J. “Influence of bacterial system on the decolourization of dye effluent under enrichment techniques” In: State Level seminar in Recent Developments in Applied microbiology, Tamil Nadu Agricultural University. Coimbatore 1998; 25-26.
18. Khalid A, Kausar F, Arshad M, Mahmood T and Ahmed I. Accelerated decolourization of reactive azo dyes under saline conditions by bacteria isolated from Arabian seawater sediment. Applied Microbiology and Biotechnology 2012; DOI 10.1007/s00253-012-3877-7.
19. Liu G, Zhou J, Wang J, Song Z and Qv Y. Bacterial decolourization of azo dyes by Rhodo pseudomonas palustris, World Journal of Microbiology and Biotechnology 2006 ;22: 1069-1074.
20. Madigan M T, Martinko J M, Parker J and Brock. Biology of microorganisms, Ed. Prentice Hall, New Jersey, USA 2000.
21. Mathur N, Bathnagar P, Nagar P and Bijarnia M K. Mutagenicity assessment of effluents from textile/dye industries of Sanganer, Jaipur (India): a case study. Ecotoxicological Environmental safety, 2005; 61:105-113.
22. Maulin P, Shah Patel K A, Nair S S and Darji A M. Bioremoval of Azo dye Reactive Red by Bacillus spp. ETL-1982. Journal of Bioremediation and Biodegradation 2013; 4: 3.
23. Ola I O, Akintokun A K, Omomowo I O and Areo V O. Aerobic decolourization of two reactive azo dyes under vary ing carbon and nitrogen source by Bacillus cereus, African Journal of Biotechnology 2010 ; 9(5) : 672-677.
24. Ponraj M, Gokila K and Zambare V. Bacterial decolourization of textile dye- Orange 3R. International Journal of Advanced Biotechnology and Resources 2011; 2: 168-177.
25. Rafii F, Franklin W and Cerniglia C E. Azoreductase activity of anaerobic bacteria isolated from human intestinal microflora. Applied Environmental and Microbiology1990; 56: 2146-2151.
26. Sahasrabudhe M and Pathade G. Decolourization of C.I. Reactive Yellow 145 by Enterococcus faecalis strain YZ66. Journal of Science and Resources 2011; 3(3):403-414.
27. Sani R K and Banerjee U C. Screening for organisms applicable to the decolourization of trimethylaniline dyes and optimization of biotranformation conditions in stirred tank reactor. Indian journal of Environment Eco planning 1999; 2: 1-9.
28. Senan R C and Abraham T E. Bioremediation of textile azo dyes by aerobic bacterial consortium. Biodegradation 2004; 15(4): 275 - 280.
29. Srividhya M R, Mary L H, Goel A M and Rangabhashiyam S. “Decolourization study of synthetic optilan red dye by Aspergillusniger”, International Journal of Recent Scientific Research 2012; 11: 301-303.
30. Wang H, Su J Q, Zheng X W, Tian Y, Xiong X J and Zheng T L. Bacterial decolourization and degradation of the reactive dye Reactive Red 180 by Citrobactersp. CK3q. International Bio deterioration and Biodegradation 2009; 63: 395-399.
31. Yatome C, Matsufuru H, Taguchi T and Ogawa T. Degradation of 4 dimethylaminobenzene-2-carboxylic acids by Pseudomonas stutzeri. Applied Microbiology Biotechnology1993; 39: 778-781.
32. Zollinger H. Colour Chemistry: Synthesis, Properties and Applications of Organic Dyes and Pigments VCH Newyork, 1987; 92-102.