IJCRR - Vol 07 Issue 02, January, 2015
CORRELATION BETWEEN BIOFILM FORMATION AND HIGHLY DRUG RESISTANT UROPATHOGENS (HDRU)
Author: V. S. Deotale, Ruchita Attal, S. Joshi, N. Bankar
Background: The biofilms have a major medical significance as they decrease the susceptibility to the antimicrobial agents.
Furthermore, the proximity of cells within a biofilm can facilitate a plasmid exchange and hence enhance the spread of antimicrobial
Objectives: The present study intends to detect biofilm formation and High Drug Resistance amongst the uropathogens and
to correlate between biofilm formation and HDRU.
Methods: This cross-sectional study was carried out over a period of two months, including 37 catheterized urinary isolate with
symptoms of UTI. Following their identification, these isolates were checked for biofilm formation by three different phenotypic
methods which includes tube adherence, Congo red agar method & tissue culture plate method. Antibiotic Susceptibility Test
was done by Kirby – Bauer Disk Diffusion method as per CLSI guidelines.
Results: Out of total 37 uropathogens isolated from catheterized urine samples, 30 (81.1%) were positive in vitro for biofilm
production & 22 (59.5%) isolates were HDRU. Maximum biofilm production was shown by E.coli (50%) , followed by Klebsiella
Keywords: Tissue Culture Plate Method (TCP), Catheter Associated Urinary Tract Infections (CAUTI), Congo red agar method
V. S. Deotale, Ruchita Attal, S. Joshi, N. Bankar. CORRELATION BETWEEN BIOFILM FORMATION AND HIGHLY DRUG RESISTANT UROPATHOGENS (HDRU) International Journal of Current Research and Review. Vol 07 Issue 02, January, 61-65
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