IJCRR - 7(5), March, 2015
Pages: 07-12
DETERMINATION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 AND ALLIED SUBTYPES IN SUDAN
Author: Mohammed A. Hammad, Karimeldin M.A. Salih, Mohammed A. Abdallah, Rashid A. Salih, Ahmed A. Mohammedani, Isam M. Elkhidir, Ayman A. Elshayeb
Category: Healthcare
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Abstract:
Introduction: Human Immunodeficiency Virus-1 (HIV-1) is the most common infection of an unresolved global disease that has had massive impact on human life since its emergence. Transmission of HIV-1 is still rapidly spreading despite identification over 33 years ago and an immense worldwide research effort to counter it.
Objectives: The aim of this study is to determine the HIV-1genotype and subtypes that cause AIDS in Sudan.
Methods: Samples were investigated and analyzed in three different laboratories; two in Sudan and the third one in Kenya, the Central Lab of Omdurman Military Hospital, Department of Microbiology, Virology Lab of Faculty of Medicine University of Khartoum and Kenya Medical Research Institute (KMERI) -Virus Research Center- Nairobi Kenya.
Results: HIV-1 was detected by RT-PCR at the virology lab and the result revealed (188) samples (90.9 %) positive for HIV- 1 (12) samples (9.1%) were negative for HIV-1. Concerning HIV-1 subtypes or clades one hundred of EDTA samples were processed at Kenya Medical Research Institute (KMERI), Using hetero-duplex Mobility Assay Technique (HMA) for env gene. Three subtypes were detected: subtype (A) (46%), Subtype (C) (33%) and subtype (D) (21%). CD4 count was estimated before antiretroviral therapy and three month after treatment, it was found that 71% were responding and 29% were not.
Conclusion: The study concluded that the detected HIV subtypes in Sudan were subtypes (A) (C) and (D). Most of the patients were responding to ARV.
Keywords: AIDS, Genotype, Heteroduplex, Kenya, Serotype, Sudan
Citation:
Mohammed A. Hammad, Karimeldin M.A. Salih, Mohammed A. Abdallah, Rashid A. Salih, Ahmed A. Mohammedani, Isam M. Elkhidir, Ayman A. Elshayeb. DETERMINATION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 AND ALLIED SUBTYPES IN SUDAN International Journal of Current Research and Review. 7(5), March, 07-12
References:
1. UNAIDS, AIDS. Epidemic update: June 2008. UNAIDS: Geneva; 2008. http://www.unaids.org/Epi2008/doc/report_pdf.html
2. Charneau P, Borman A, Quillent C, et al. Isolation and envelope sequence of a highly divergent HIV-1 isolate: definition of a new HIV-I group. Virology 1994; (2): 59 247-253.
3. Fauci AS, Lane HC. Human immunodeficiency virus disease: AIDS and related disorders. Harrison’s Principles of Internal Medicine 16th Edition. New York, McGraw-Hill Medical Publications Division 2005.
4. UNGASS HIV/AIDS in Northern Sudan. UNGASS Report January 2008. World Health Organization, WHO report 2014.
5. Graeme JS, Irvine SS, Scott M, Kelleher AD, et al. Strategies of care in managing HIV. In Managing HIV. Sydney: Australasian Medical Publishing Company Limited 1997.
6. World Health Organization, WHO Case Definitions of HIV for Surveillance and Revised Clinical Staging and Immunological Classification of HIV-related disease in Adults and Children, August 7, 2006.
7. UNAIDS. Epidemiological Fact Sheets on HIV/AIDS and Sexually Transmitted Infections: Yemen. Geneva: UNAIDS; 2004a [Accessed 12 May 2006]. Online at: http://data.unaids.org/Publications/FactSheets01/yemen_EN.pdf.
8. Louise Lambert, M. Ed., D, HIV and development challenges in Yemen: which grows fastest? Oxford journal. 2007; volume22,issue 1p60-62.
9. WHO-UNAIDS Network for HIV isolation and characterization, Hemelaar J, Gouwsb E, Ghysb DP: Global trends in molecular epidemiology of HIV-1 during 2000–2007. AIDS 2011; (25): 679–689.
10. Lihana WR, Ssemwanga D, Abimiku A, Ndimbi A: Update on HIV-1 diversity in Africa: A decade in review. AIDS Rev 2012; 14:83–100.
11. Philippe D and Charles B. The HIV/AIDS Epidemic in SubSaharan Africa in a Historical Perspective Philippe Denis and Charles Becker (eds) Online edition, October 2006; pp. 35-54.
12. Osmanov S, Pattou C, Walker N, et al: WHO-UNAIDS Network for HIV Isolation and Characterization: Estimated global distribution and regional spread of HIV-1 genetic subtypes in the year 2000. J Acquir Immune Defic Syndr 2002; 29:184-190.
13. Geretti AM: HIV-1 subtypes: epidemiology and significance for HIV management. Curr Opin Infect Dis 2006; 19(1):1- 7.
14. Khamadi S., Ocheing W., Raphael C., et al, HIV Type 1 Subtypes in Circulation in Northern Kenya. AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 21, Number 9, 2005, pp. 810–814.
15. Bobkov AF, Kazennova EV, Selimova LM et al. “Temporal trends in the HIV-1 epidemic in Russia: predominance of subtype A”. J. Med. Virol. 2004;74 (2): 191–6. doi:10.1002/ jmv.20177. PMID 15332265.
16. Goudsmit, Jaap. Viral Sex; The Nature of AIDS. Oxford University Press. New York, New York, 1997. Pg. 51-58. Retrieved May 25, 2008.
17. Robertson DL, Hahn BH, Sharp PM. “Recombination in AIDS viruses”. J. Mol. Evol. 1995,40 (3): 249–59. doi:10.1007/ BF00163230. PMID 7723052.
18. UNAIDS and Government of South Sudan. Global AIDS response report, 2001; (2): 44- 46.
19. U.S. Department of Health and Human Services’ Guidelines for the Use of Antiretroviral Agents in HIV-1-Infected Adults and Adolescents (available at http://aidsinfo.nih. gov/guidelines).
20. Hierholzer M, Graham RR, ElKhidir I, et al. HIV Type 1 Strains from East and West Africa are intermixed in Sudan. AIDS Res Hum Retroviruses. 2002; 18 (15): 1163-6.
21. Centers for Disease Control and Prevention. Vital signs: HIV prevention through care and treatment—United States. MMWR Morb Mortal Wkly Rep. 2011; 60(47):1618- 1623.Availableat http://www.ncbi.nlm.nih.gov/pubmed/22129997.
22. Cozzi Lepri A, Katzenstein TL, Ullum H, et al. The relative prognostic value of plasma HIV RNA levels and CD4 lymphocyte counts in advanced HIV infection. AIDS. 1998;12:1639-43.
23. Kim S, Els D, Nancy D et al. A sensitive in-house RT-PCR genotyping system for combined detection of plasma HIV-1 and assessment of drug resistance. Journal of Virological Methods 133. 2006; 137–145.
24. Vidal N, Mulanga C, EdidiBazepeo S, et al. Distribution of HIV-1 Variants in the Democratic Republic of Congo Suggests Increase of Subtype C in Kinshasa Between 1997 and 2002. J AIDS. 2005; 40 (4): 456-62.
25. Gardner EM, McLees MP, Steiner JF, et al. The spectrum of engagement in HIV care and its relevance to test-and-treat strategies for prevention of HIV infection. Clin Infect Dis. 2011;52(6):793-800. Available at http://www.ncbi.nlm. nih.gov/pubmed/21367734.
26. Janssens W, Heyndrickx L, Fransen K, et al: Genetic variability of HIV type 1 in Kenya. AIDS Res Hum Retroviruses 1994;10:1577±1579.
27. Poss M, Gosink J, Thomas E, et al: Phylogenetic evaluation of Kenyan HIV type 1 isolates. AIDS Res Hum Retroviruses 1997;13: 493±499.
28. Zachar V, Goustin AS, Zacharova V, et al: Genetic polymorphism of envelope V3 region of HIV type 1 subtypes A, C, and D from Nairobi, Kenya. AIDS Res Hum Retroviruses 1996;12:75±78.
29. Ann A, Fredrik G, Anna Li, et al. Impact of HIV type1 genetic subtype on the outcome of antiretroviral therapy.AIDS research and human retrovirus march 2005;21(3) 227.
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