IJCRR - Vol 07 Issue 09, May, 2015
CAFFEINE EXTRACTION AND CHARACTERIZATION
Author: Pradeep S., G. N. Rameshaiah, Hadagali Ashoka
Category: General Sciences
Caffeineextracted and characterised from tea (black) leaves and coffee beans. Isolation was done by liquid-liquid extraction using di-chloromethane as an extracting agent. This extraction was done in four steps: steeping, evaporation, liquid-liquid extraction and recrystallization. The recrystallization was done using anhydrous sodium sulphate.The technique used for purity analysis and characterisation were: High performance liquid chromatography, Differential scanning calorimeter, Fourier transform infrared spectroscopyand Melting point. First, the analysis was done using melting point analysis. The melting point of caffeine
extracted from coffee beans and tea leaves was found to be 238°C. The absorption bands were compared with that available in literature and were found to be similar. Further, the purity check was done using High performance liquid chromatography method.Effective characterization of caffeine was achieved by determining Infrared spectrum, and employing a melting point apparatus and differential scanning calorimeter. The purity showed that the results that the extracted coffee was 90% pure. Further improvements in extraction efficiency will increase the yield and minimize wastage.
Keywords: Caffeine, Methyl xanthine, Theophylline, Differential scanning calorimeter, Fourier transform infrared spectroscopy, High performance liquid chromatography
Pradeep S., G. N. Rameshaiah, Hadagali Ashoka. CAFFEINE EXTRACTION AND CHARACTERIZATION International Journal of Current Research and Review. Vol 07 Issue 09, May, 16-19
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