Anti-diabetic Effect of Ethanolic Extracts of Cissus Quadrangularis linn Fruits and Michelia Champaea Leaves in Alloxan Induced Hyperglycaemic Rats and in 3T3L1 Cell Lines

Jasti Deepthi; D. V. R. N Bhikshapathi; Palanati Mamatha; Puttaswamy Nirmala

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IJCRR - 14(13), July, 2022

Pages: 52-59

Anti-diabetic Effect of Ethanolic Extracts of Cissus Quadrangularis linn Fruits and Michelia Champaea Leaves in Alloxan Induced Hyperglycaemic Rats and in 3T3L1 Cell Lines

Author: Jasti Deepthi, D. V. R. N Bhikshapathi, Palanati Mamatha, Puttaswamy Nirmala

Category: Healthcare

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Abstract:

Background: Diabetes is a metabolic disorder that results in increased blood sugar. This study came up with a search for alternative medicines with no or fewer side effects for antidiabetic therapy. Materials and methods: The plants are extracted and screened for the phytochemical study of crude extracts. Anti-diabetic activity in alloxan-induced hyperglycaemic rats, in a single-dose study, multiple-dose treatment was investigated. In vitro cellular assay using 3T3L1 cell line was performed to check the cell viability with increasing plant extract treatment. Results: Phytochemical investigation reveals the presence of alkaloids, flavonoids, saponins, tannins, steroids, triterpenoids, carbohydrates, and glycosides in both plant extractions. In acute toxicity studies, no mortality was observed with either of the extracts even at a dosage of the level of 5000 mg per kg of body weight. The ethanolic extracts showed a noteworthy decline in blood glucose level in alloxan-induced rats in both single and multiple dosage methods. Significant changes were observed in the serum glucose and body weight from day 0 to day 14. The cell viability of the extracts was also comparable with the standard. Conclusion: Our results report that CQ fruits and MC leaves have potent antidiabetic action and on further studies, can be a credible resource in antidiabetic therapy.

Keywords: Cissus Quadrangularis Linn Fruits, Michelia Champaea Leaves, Anti-diabetic activity, 3T3L1 cell line, Cyototoxicity, Wistar rats.

DOI URL: https://doi.org/10.31782/IJCRR.2022.141309

Citation:

Jasti Deepthi, D. V. R. N Bhikshapathi, Palanati Mamatha, Puttaswamy Nirmala. Anti-diabetic Effect of Ethanolic Extracts of Cissus Quadrangularis linn Fruits and Michelia Champaea Leaves in Alloxan Induced Hyperglycaemic Rats and in 3T3L1 Cell Lines International Journal of Current Research and Review. 14(13), July, 52-59

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