IJCRR - Vol 07 Issue 19, October, 2015
IDENTIFICATION OF UROVIRULENT MARKERS IN UROPATHOGENIC ESCHERICHIA COLI
Author: D. Vijaya Bharathi, R. Lakshmi Kumari, G. Rajya Lakshmi, Supriya Panda
Context: Escherichia coli is the most frequent urinary pathogen isolated from uncomplicated urinary tract infection. These isolates express chromosomally encoded virulence markers.
Aims: The present study was designed to determine the urovirulence factors of E.coli isolated from the patients of urinary tract infection and to study their antimicrobial susceptibility pattern.
Methods and Material: The study was conducted in the department of microbiology, Rangaraya Medical College, Kakinada, Andhra Pradesh, from October 2010 to September 2011.One hundred and fifty E.coli strains isolated from urine samples and fifty faecal isolates were studied for 1) alpha haemolysin on 5% sheep blood agar, 2) mannose resistant haemagglutination,3) cell surface hydrophobicity, 4) antibiotic susceptibility testing by Kirby-Bauyer’s disc diffusion method.
Results: Among 150 E.coli isolates from urine tested 82 (54%) and out of 50 E.coli isolates from stool (control), 10 (20%) were positive for virulence markers. In the urinary isolates, the most common virulent marker was Haemolysin 59 (39%), followed by Mannose Resistant Haemagglutination (MRHA) 47(31%) and Cell Surface Hydrophobicity (CSH) 43 (28%). In control group, the occurrence of Haemolysin was 3 (6%), MRHA 6 (12%), CSH 9(18%). The difference between cases and controls for Haemolysin and MRHA were significant. (p<0.05 and P<0.005 respectively). Ninety two percent isolates were sensitive to amikacin and high resistance was seen for ampicillin (91%).
Conclusions: More number of uropathogenic strains of E. coli expressed urovirulent markers than faecal strains. Majority of the isolates were found to be resistant to routinely used antibiotics.
Keywords: Uropathogenic E. coli, Urovirulent markers, Urinary tract infection
D. Vijaya Bharathi, R. Lakshmi Kumari, G. Rajya Lakshmi, Supriya Panda. IDENTIFICATION OF UROVIRULENT MARKERS IN UROPATHOGENIC ESCHERICHIA COLI International Journal of Current Research and Review. Vol 07 Issue 19, October, 51-54
1. Steadman R., and Topley N. 1998. The virulence of Escerichia coli in Urinary tract. Chapter 3 In: Urinary tract infections, 1st ED, Brumfitt W. JeremyMT, Hamilton Miller, Eds, (Chapmanand Hall publication, London). 3741.
2. Raksha R., and Srinivasa, N. 2003. Occurrence and characterization of uropathogenic E.coli in urinary tract infections, Indian. J. Med. Microbiol. 21 (2): 102–107.
3. Struve C., and Krogfelt K.A. 1999. In vivo detection of Escherichia coli type-1 fimbrial expression and phase variation during experimental urinary tract infection. Microbiol. 145:2683-90
4. Cowan and Steel’s Manual for identification of Medical bacteria, 3rd ed. Eds. Barrow Gl, Feltham RKA. Eds. (University of Cambridge) 1993.
5. Cavalieri S.J., Bohach GA and Snyder, I.S. 1984. Escherichia coli alpha haemolysin: characteristics and probable role in pathogenicity. Microbiol. Rev. 48:326-343.
6. Duguid J.P., Clegg S and Wilson MJ.1979. The fimbrial and non-fimbrial haemagglutinins of Escherichia coli . J. Med. Microbiol. 12:213-27.
7. Lindahl M, Faris A, Wadstrom T, Hjerten S. A new test based on salting out to measure relative surface hydrophobicity.
8. Yasmeen Kausar, Sneha K Chunchanur ,Shoba D Nadagir, LH Halesh and MR Chandrasekhar. Virulence factors, Serotypes and antimicrobial susceptibility pattern of Escherichia coli in urinary tract infections.Al Ameen Journal of Medical Sciences(2009) 2(1):47-51
9. Smith,H.W,1963.The hemolysin of Escherichia coli, J Pathol Bact.85:197-211
10. Prabhat Ranjan, K., Neelima Ranjan, Arindam Chakraborthy, D R Arora. 2010. An Approach to Uropathogenic Escherichia coli in Urinary Tract Infections. J. Lab. Physic.2, issue2:70-73.
11. Jonson, J.R. Virulence factors in Escherichia coli urinary tract infections. Clin. Microbiol Rev. 1991. 4:81-128.
12. Mudd S, Mudd EBH. The penetration of bacteria through capillary spaces IV. A kinetic mechanism in interfaces J Exp Med 1924;40:633-45.