IJCRR - Vol 07 Issue 24, December, 2015
ISOLATION AND SPECIES IDENTIFICATION OF CANDIDA ISOLATED FROM PATIENTS OF VULVOVAGINAL CANDIDIASIS IN A TERTIARY CARE HOSPITAL
Author: Twinkle Gandhi, Manish G. Patel, Mannu Jain
Introduction: An increase in the predisposing conditions in recent years has resulted in an increasing incidence of Candida infections.The accurate species identification of Candida is important for the treatment, as not all species respond to the same treatment and also because of the problem of anti-fungal resistance in certain species.Therefore, the species level identification of the Candida isolates, along with their anti-fungal susceptibility patterns can greatly influence the treatment options for the clinician. Objective: - To detect prevalence of Candida in patients with vaginal discharge. - To identify species of Candidaisolates. Methods: This study included 410 women with abnormal vaginal ischarge. Two high vaginal swabs were collected and sent immediately to the laboratory. All vaginal samples were stained with gram’s stain and oval budding yeast cells were identified as Candida. Second swab was inoculated on Sabouraud’s Dextrose agar and Candida isolates(n=122)were identified at species level by battery of various tests like GTT,Corn meal agar, Carbohydrate fermentation, Carbohydrate assimilation and CHROM agar inoculation. Results: Out of 410 samples Candida were isolated in 122 samples. Amongst the 122 Candida isolates highest was C.albicans 66.39%,followed by C.glabrata 15.65%,C.tropicalis 9.85%, C.parapsilosis 4.91%, C.krusei 2.4% and least wasC.guilliermondii 0.8%.In pregnancy and OCP users maximum isolates were of C.albicans(78.04% and 55.55%,respectively) were as in diabetic patients C.glabrata (47.05%) was predominant isolate,while in HIV positive patients all isolates wereC.albicans (100%). Conclusions: In our study the prevalence of VVC is 29.75%.Commonest species found was C.albicans 66.39%.VVC is more common in females with associated risk factors like pregnancy, diabetes, HIV and OCP users.Commonest species in pregnancy, OCP users and HIV patients wasC.albicans whileC.glabratawas more common in diabetic patient.
Keywords: Vulvovaginal candidiasis (VVC), Candida Spp., Germ tube test (GTT), CHROM agar
Twinkle Gandhi, Manish G. Patel, Mannu Jain. ISOLATION AND SPECIES IDENTIFICATION OF CANDIDA ISOLATED FROM PATIENTS OF VULVOVAGINAL CANDIDIASIS IN A TERTIARY CARE HOSPITAL International Journal of Current Research and Review. Vol 07 Issue 24, December, 06-12
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