IJCRR - Vol 08 Issue 09, May, 2016
OXIDATIVE STRESS BIOMARKERS IN RATS EXPOSED TO BROMOXYNIL
Author: Ahmed K. Salama, Khaled A. Osman, Ahmed S. El-Bakary, Maher S. Salama
Aim: The present study was designated to evaluate the oxidative stress biomarkers in male rat following oral repetitive administration of 0.1 LD50 of the herbicide bromoxynil. Methodology: Animals were orally received four doses of 0.1 LD50 of bromoxynil every other day. Twenty-four hours after the last oral dosing, all rats were killed by decapitation. Blood, brain, liver, and kidneys were taken for determination of TBARS, lactic dehydrogenase, catalase and alkaline phosphatase. Results: TBARS were found to be significantly increased in the liver, kidneys and brain where they were 390.20, 293.80, and 287.03% of control, respectively. In case of serum it was insignificantly increased to 162.88% of control value. Lactic dehydrogenase activity was significantly enhanced in serum and liver comparing with the control values (119.49 and 114.12%) and insignificantly enhanced in kidneys and brain (105.17 and 107.40%). Catalase activity was increased in all tissues where the enhancement was significant in both of serum and liver (122.68 and 119.99%, respectively) and insignificant in case of kidneys and brain (112.55 and 105.12%, respectively). Alkaline phosphatase activity in serum, liver, kidneys and brain was found to be elevated. These values were significant in liver and kidneys (113.47 and 121.14%, respectively) while they were insignificant in serum and brain (109.91 and 114.46%, respectively). Conclusion: Therefore, the herbicide bromoxynil could produce significant alteration in the lipid peroxidation and activities of some antioxidant enzymes and producing cellular oxidative damage in male rats following repetitive oral dosing.
Keywords: Bromoxynil, Oxidative stress, Rat, Biomarkers, Antioxidant, Enzymes
Ahmed K. Salama, Khaled A. Osman, Ahmed S. El-Bakary, Maher S. Salama. OXIDATIVE STRESS BIOMARKERS IN RATS EXPOSED TO BROMOXYNIL International Journal of Current Research and Review. Vol 08 Issue 09, May, 01-06
1. Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S.; Rezaiee, A., 2004. Pesticides and oxidative stress: a review. Med. Sci. Monit., 10: 141–147.
2. Aebi, H., 1984, Catalase in vivo. Methods Enzymol. 105, 121- 126.
3. Akhgari, M. , Abdollahi, M. , Kebryaeezadeh, A., Hosseini, R. Sabzevari , O. 2003, Biochemical evidence for free radical-induced lipid peroxidation as a mechanism for subchronic toxicity of malathion in blood and liver of rats. Hum Exp Toxicol., 22(4):205-11.
4. Astiz, M., Arnal, N., de Alaniz, M.J. and Marra, C.A. , 2011, Occupational exposure characterization in professional sprayers: Clinical utility of oxidative stress biomarkers. Environ. Toxicol. Pharmacol., 32(2):249-258.
5. Banerjee, B.D., Ahmed, R.S., 2001. Pesticide-induced oxidative stress: perspectives and trends. Rev Environ Health.16(1):1-40.
6. Bhanti, M., Taneja, A., 2007, Contamination of vegetables of different seasons with organophosphorus pesticides and related risk assessment in northern India. Chemosphere, 69(1):63-68.
7. Costat Program, 1986, Version 2, Cohort Software.
8. Farber, J.L., Kyle, M.E. , Coleman, J.B., 1990, Biology of disease: Mechanisms of cell injury by activated oxygen species. La. Inves. 62:670-679.
9. Franco, R., Sanchez-Olea, R., Reyes-Reyes, E.M., Panayiotidis, M.I., 2009, Environmental toxicity, oxidative stress and apoptosis: ménage a trois. Mutat. Res., 674:3-22.
10. Franco, R., Schoneveld, O.J., Pappa, A., Panayiotidis, M.I., 2007, The central role of glutathione in the pathophysiology of human diseases. Arch. Physiol. Biochem., 113:234-258.
11. George, N., Bowers, Jr., McComb, R.B., 1966. A Continuous Spectrophotometric Method for Measuring the Activity of Serum Alkaline Phosphatase. Clinical Chemistry, 12(2): 70-89.
12. Hermes-Lima, M., 2004, Oxygen in biology and biochemistry: role of free radicals. In: Storey, K.B. (Ed.), Functional metabolism: Regulation and adaptation. John Wiley and Sons, New York, pp. 319-368.
13. Jaiswal, S.K., Siddiqi, N.J., Sharma, B., 2014, Carbofuran induced oxidative stress mediated alterations in Na+ -K+ -ATPase activity in rat brain: amelioration by vitamin E. J. Biochem. Mol. Toxicol., 28(7):320-7.
14. Krieger, R., Doull, J., Ecobichon, D., Gammon, D., Hodgson, E., Reiter, L. Ross, J. 2001, Handbook of Academic Toxicology Principles, Academic Press.
15. Lowry O.H., Rosebrugh, N.J., Farr , A.L. , Randall, R.J. , 1951, Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265- 275.
16. Moss, D.W., Henderson, A.R. , Kachmar, J.R. , 1986, Enzymes. In: Textbook of Clinical Chemistry (N.W. Tietz, Ed.), W.B. Saunders, Philadelphia, PA. pp:691-663.
17. Muniz, J.F., McCauley, L., Scherer, J., Lasarev, M., Koshy, M., Kow, Y.W., Nazar-Stewart, V., Kisby, G.E., 2008, Biomarkers of oxidative stress and DNA damage in agricultural workers: a pilot study. Toxicol. Appl. Pharmacol., 227(1):97-107
18. Osman, K.A., Aly, N.M. , Salama, A. K. , 2000, The role of vitamin E and glutathione as antioxidants in the protection of oxidative stress induced by paraquat and diquat in female rats. Alex. Sci. Exch., vol 21 : 247-259.
19. Osman, K.A.,1999, Lindane, chlorpyrifos and paraquat induced oxidative stress in female rats. Alex.J. Res., 44:345-355.
20. Peluso, M., Srivatanakul, P., Munnia, A., Jedpiyawongse, A., Ceppi, M., Sangrajrang, S., Piro, S., Boffetta, P. 2010, Malodialdehyde-Deoxyguanosine adducts among workers of Thai industerial estate and nearby residents. Environ. Health Perspect. 118(1), 55-59.
21. Poljsak, B., Fink, R. , 2014, The protective role of antioxidants in the defence against ROS/RNS-mediated environmental pollution. Oxid Med Cell Longev., 2014:671539.
22. Roberts, T. R., Huston, D. H., Lee, P. W., Nicholls, P. H., Plimmer, J. R., 1998, Metabolic pathways of agrochemicals. Part 1, Herbicides and plant growth regulators. The Royal Society of Chemistry, Cambridge, UK.
23. Ryter, S.W., Kim, H.P., Hoetzel, A., Park, J.W., Nakahira, K., Wang, X., Choi, A.M., 2007, Mechanisms of cell death in oxidative stress. Antioxid. Redox Signal. 9:49-89
24. Salama A.K., Osman, K. A., Ahmed, N. S. , Soliman, S.A. ,2005, Oxidative stress induced by different pesticides in the land snails Helix aspersa. Pak. J. Biol. Sci 8 (1), 92-96.
25. Salama, A.K., Osman, K. A., Omran, O.A. , 2013, Pesticidesinduced oxidative damage: Possible in vitro protection by antioxidants. Journal of Toxicology and Environmental Health Sciences, 5(5):79-85.
26. Shaffi, S.A., 1980, Thiodon toxicity: non-specific phosphomonoesterases in nine freshwater teleosts. Toxicol. Letters, 6:339- 347.
27. Shvedova, A.A., Yanamala, N., Murray, A.R., Kisin, E.R., Khaliullin, T., Hatfield, M.K., Tkach, A.V., Krantz, Q.T.,Nash, D., King, C., Gilmour , M.I., Gavett, S.H. 2013, Oxidative Stress, Inflammatory Biomarkers, and Toxicity in Mouse Lung and Liver after Inhalation Exposure to 100% Biodiesel or Petroleum Diesel Emissions. J. Toxicology and Environmental Health A, 76 (15): 907-921.
28. Tabatabaie T, Floyed, R.A., 1996, Inactivation of glutathione peroxidase by benzaldehyde. Toxicol. Appl. Pharmacol. 141: 389-393.
29. Tomlin, C.D. (2001): “The pesticide manual” 12th ed, British Crop Protection.
30. Uchiyama, M. and Mihara,M., 1978, Determination of malondialdehyde precuesor in tissue by thiobarbaturic acid test. Anal. Biochem., 86:271-278.
31. West, J.D., Marnett, L.J., 2006, Endogenous reactive intermediates as modulators of cell signaling and cell death. Chem. Res. Toxicol., 19:173-194.