IJCRR - 8(19), October, 2016
Pages: 06-11
ASSESSMENT OF SALIVARY ENZYMES-ALANINE AMINOPEPTIDASE(ALAP) AND DIPEPTIDYL PEPTIDASE IV (DPP IV) IN PATIENTS WITH CHRONIC PERIODONTITIS
Author: S. Rajasekar, V. Ramasubramanian, Sethupathi
Category: Healthcare
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Abstract:
Introduction: Alanine Aminopeptidase (ALAP) and dipeptidylpeptidase (DPP IV) are proteolytic enzymes released in the periodontal tissues from leukocytes, host cells and microorganisms. Both enzymes play an important role in collagen degradation and destruction of periodontal tissues. Therefore, detection of these enzymes might be useful in the diagnosis of periodontal disease.
Aim: To assess the salivary levels of ALAP and DPP IV in periodontally healthy and those with chronic Periodontitis and to correlate the enzyme levels with the severity of disease. A prospective comparative case control study was done on 60 systemically healthy patients in the age group of 25-55 and was divided into four groups with 15 patients in each as Periodontally healthy, Mild, Moderate and Severe Periodontitis. Methodology: Parameters like Plaque index, Gingival bleeding index, Probing pocket depth and clinical attachment loss were recorded at baseline and unstimulated whole saliva was collected from each patient and quantified for levels of ALAP and DPP IV using spectroscopic quantification method. Statistical analysis used ANOVA, Students t test and Pearson’s correlation coefficient analysis was used.
Results: The salivary levels of ALAP and DPP IV were found to be higher in patients with Periodontitis than p healthy controls and were higher in severe (42.34±8.96) (8.41±1.44) and moderate group) (25.46±6.79) (5.17±1.31) than mild Periodontitis group) (15.83±4.54) (3.69±1.00) which was statistically significant.
Discussion and Conclusion: ALAP and DPP IV were higher in saliva of patients with chronic Periodontitis and positive correlation existed between the levels of these enzymes with the severity of Periodontitis.
Keywords: Alanine aminopeptidase (ALAP), Dipeptidylpeptidase IV (DPP IV), Saliva, Periodontitis
Citation:
S. Rajasekar, V. Ramasubramanian, Sethupathi. ASSESSMENT OF SALIVARY ENZYMES-ALANINE AMINOPEPTIDASE(ALAP) AND DIPEPTIDYL PEPTIDASE IV (DPP IV) IN PATIENTS WITH CHRONIC PERIODONTITIS International Journal of Current Research and Review. 8(19), October, 06-11
References:
1 Nurdan ozmeric. Advances in periodontal disease markers. Clinica chimica Acta 2004; 343:1-16.
2 Serenay Elgum, Nurdan ozmeric, Selda Demirtas. Alanine aminopeptidase and Dipeptidyl peptidase IV in saliva ; a possible role in periodontal disease. Clinica Chimica Acta 2000;298:187
3 Edgar WM. Saliva, its secretion, composition and functions. British dental journal 1992;172:305
4 Wikipedia.com. Alanine aminopeptidases and Dipeptidyl peptidase IV.
5 Piyamas Aemaimanan, Nison sattayasai, Nawarat waracaswapati, Waranuch pitiphet, Saengsome prajaneh. Alanine aminopeptidase and Dipeptidylpeptidase IV in saliva of chronic Periodontitis patients. J Periodontol 2009; 80:1809-1814.
6 Michael G Newman, Henry H Takei, Fermin A Carranza. Clinical Periodontology 9th edition; WB Saunders company, The Curtis centre independence square west Philadelphia, PA 19106 2003 ISBN 0-7216-8331-2, An imprint of Elsevier Science.p121-149 ,p190-192.
7 Silness P, Loe H . Periodontal disease in pregnancy; correlation between oral hygiene and periodontal condition. Acta odontol scand 1964; 2: 121-135.
Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J 1975; 25:229-235.
Sidorowiez, Wen Cheng Hsia, Olgomaslej zownir, Behal FJ. Multiple molecular forms of human alanine aminopeptidase ; Immunohistochemical properties. Clinica Chimica Acta 1980; 107: 245-256.
Toshikaru Nagatsu, Masami Hino, Hiroshi Fuyamada, Taro Hayakawa, Yasuo Nakagawa. New chromogenic substrates for X-prolyl dipeptidyl aminopeptidase. Analytical Biochemistry 1976; 74:466-477
Eley BM, Cox SW. Cathepsin, elastase, trypsin and dipeptidylpeptidase in GCF ; Correlations with clinical parameters in untreated chronic Periodontitis patients. J Periodont Research 1992; 27: 62-69
Abiko Y, Hayakawa M, Murai S, Takiguchi H. Glycyl prolyl dipeptidyl aminopeptidase from Bacteroides gingivalis. J Dent Research 1985;64 :106-111
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