IJCRR - Vol 08 Issue 10, May, 2016
QUANTIFICATION OF ANTIOXIDANTS IN UNDERUTILIZED VEGETABLE LEAVES
Author: Mary Jenefer Sharmila P., Dorothy Jaganathan, Kumaravel S.
Introduction: Green leafy vegetables are an excellent source of bioactive compounds beyond human nutrition. There are many underutilized vegetable leaves which are equally a power house of bioactive compounds with reference to antioxidants. Much literature confirms that brassicca vegetables are high in antioxidants but the antioxidant studies these vegetable leaves are scarce as they are underutilized and neglected hence the present study was framed.
Aim: To analyze the total antioxidants, total phenols and total flavonoids in the leaves of cauliflower radish and beetroot leaves. Methodology: Total antioxidants were analysed using Dpph method and phenols by Folin-Ciocalteu method and Total flavonoids were determined spectrophotometrically. Results: The observations were read in triplicates. The data was analyzed statistically showing the presence of total antioxidants, total phenols and total flavonoids in leaves of cauliflower radish and beetroot which can a therapeutic role in the health of humans. Conclusion: Hence the present study reveals the benefits of these underutilized leaves which are packed with antioxidants that can prevent from all degenerative diseases and these antioxidant leaves can be also treated in formulation of functional foods and in food and health industry.
Keywords: Underutilized vegetable leaves, Total antioxidants, Total phenols, Total flavonoids, Degenerative diseases
Mary Jenefer Sharmila P., Dorothy Jaganathan, Kumaravel S.. QUANTIFICATION OF ANTIOXIDANTS IN UNDERUTILIZED VEGETABLE LEAVES International Journal of Current Research and Review. Vol 08 Issue 10, May, 01-04
1. Papas, A. M. In phytochemicals in Nutrition and Health, Meskin, M. S., Bidlack, W. R., Davies, A. J. and Omaye. S. T. New York: CRC Press, (2002) 61-78.
2. AchikanuC.E.et.al Determination of the vitamin and mineral composition of common leafy vegetables in south eastern Nigeri, Int.J.Curr.Microbiol.App.Sci (2013) 2(11): 347-353.
3. Gulcin,. M.E. Buyukokurolu, M. Oktay and O. Kufreviolu (2002), on the in vitro antioxidant properties of melatonin. J. Pineal Res.33: 167-171.
4. Ghosal.S. VK Tripathi; S Chaeruhann. Indian Journal o of Chemistry, 1996, 35, 941-948.
5. ER Sherwin; AL Brancn; PM Davidson; Salmincn S. Food Additives. Marvel Dekker Inc, New York, 1990, 139-193.
6. Gulcin. Antioxidant activity of caffeic acid (3,4dihydroxycinnamic acid).Toxicology217:213-220, 2006
7. Liyana-Pathirana, C.M. and F. Shahidi Antioxidant properties of commercial soft and hard winter wheats (Triticum aestivum L.) and their milling fractions. J. Sci. Food Agric (2006)86: 477-485.
8. Deng, G.-F.; Lin, X.; Xu, X.-R.; Gao, L.-L.; Xie, J.-F.; Li, H.-B. Antioxidant capacities and total phenolic contents of 56 vegetables. J. Funct. Foods (2012) 5, 260–266.
9. Li, X.L.; Zhou, A.G.; Han, Y. Anti-oxidation and antimicroorganism activities of purifi cation polysaccharide from Lygodium japonicum in vitro. Carbohydr. Polym. (2006) 66, 34–42.
10. Schofi eld, P.; Mbugua, D.M.; Pell, A.N. Analysis of condensed tannins: A review. Anim. Feed Sci. Tech. 2001, 91, 21–40.
11. Farmacopeia Brasileira, 5th ed.; Agência Nacional de Vigilância Sanitária: Brasília, Brazil, 2010
12. Anna Pkal and Krystyna Pyrzynska, Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay, Food Anal.methods 2014, 7:1776-1782 , DOI 10.1007/s12161-014-9814-x
13. Di Matteo, V. Esposito, E. Biochemical and therapeutic effects of antioxidants in the treatment of Alzheimer`s disease, Parkinson`s disease, and amyotrophic lateral sclerosis. Curr. Drug Targets CNS Neurol. Disord. 2003, 2, 95–107.
14. Singh S, Singh RP. In vitro methods of assay of antioxidants: An overview. Food Rev Int. 2008; 24(4):392–415. Doi: 10.1080/87559120802304269.
15. Cui K, Luo X, Murthy MRV. Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants. Prog Neuropsychopharmacol Biol Psych. 2004; 28:771–799. Doi: 10.1016/j.pnpbp.2004.05.023. [PubMed] [Cross Ref]
16. Clifford, M.N. (2000). Chlorogenic acids and o t h e r cinnamates nature, occurrence, dietary b u r d e n , absorption and metabolism.Journal of the Science of Food and Agriculture, 80, 1033–1043.
17. Pourcel, L.; Routaboul, J.M.; Cheynier, V.; Lepiniec, L.; Debeaujon, I. Flavonoid oxidation in plants: From biochemical properties to physiological functions. Trends Plant Sci. 2007, 12, 29–36.