IJCRR - Vol 08 Issue 11, June, 2016
EFFECT OF ISOPROTURON TOXICITY ON TESTIS OF ALBINO RATS
Author: Archana Rani, Anita Rani, Rakesh Kumar Verma, Arvind Kumar Pankaj
Objectives: As pesticides hold a unique position among environmental contaminants; the objective of the present study was to evaluate the toxic effects of Isoproturon, a substituted phenylurea herbicide on testis of albino rats.
Methods: The compound, suspended in groundnut oil, was administered orally to rats for 60 days at the doses of 400 mg/kg and 800 mg/kg body weight for two groups respectively. Body weight, testis weight and seminiferous tubular diameter were measured. Histological study was done on haematoxylin- eosin stained 5μm thick paraffin sections.
Results: Decrease in weight gain and seminiferous tubular diameter was observed in experimental rats which was highly significant. The difference in testis weight was not significant statistically in experimental group as compared to control group. Light microscopic study showed degenerative changes in the testis.
Conclusions: Degenerative changes in the gametogenic cells indicate adverse effects on the functional ability of these cells and are highly conclusive of reproductive toxicity caused by isoproturon.
Keywords: Isoproturon, Testis, Seminiferous tubules, Leydig cells
Archana Rani, Anita Rani, Rakesh Kumar Verma, Arvind Kumar Pankaj. EFFECT OF ISOPROTURON TOXICITY ON TESTIS OF ALBINO RATS International Journal of Current Research and Review. Vol 08 Issue 11, June, 23-27
1. Lorenz, Eric S. Potential Health Effects of Pesticides. Ag Communications and Marketing. 2009; 1-8.
2. Daniels JL, Andrew FO, David AS. Pesticides and Childhood Cancers. Department of Epidemiology, School of Public Health, University of North Carolina-Chapel Hill, Chapel Hill, NC. Web of Science. 1997; 105.
3. Hodgson, Ernest, Levi, Patricia E. Pesticides: An important but underused model for the environmental health sciences. Environmental Health Perspectives Supplements. Academic Search Premier. 1996; 104 Suppl 1.
4. Rabeh NM. Effect of red beetroot (Beta vulgaris L.) and its fresh juice against carbon tetrachloride induced hepatotoxicity in rats. World Applied Sciences Journal. 2015; 33 (6): 931-938.
5. Guidelines for care and use of animals in scientific research. Indian National Science Academy New Delhi. S. K. Sahni, Executive Secretary, Indian National Science Academy, New Delhi; 2000.
6. Dikshith TS, Raizada RB, Srivastava MK. Dermal toxicity to rats of isoproturon technical and formulation. Vet Hum Toxicol. 1990; 32(5):432-434.
7. Sarkar SN, Gupta PK. Fetotoxic and teratogenic potential of substituted phenylurea herbicide, isoproturon, in rats. Indian J Exp Biol. 1993; 31 (3):280-282.
8. Sarkar SN, Chattopadhyay SK, Majumdar AC. Subacute toxicity of urea herbicide, isoproturon, in male rats. Indian J Exp Biol. 1995; 33:851-856.
9. Sarkar SN, Majumdar AC, Chattopadhyay SK. Effect of isoproturon on male reproductive system: clinical, histological and histoenzyonological studies in rats. Indian J Exp Biol. 1997; 35(2):133-138.
10. Griffin JE, Wilson JD. Harrison’s principles of internal medicine. Wilson JD, Braunwald E, Isselbacher KJ, Petersdrof RG, Martin JB, Fauci AS, Root KK. Mcgraw Hill, New York; 1991.
11. Lox CD. The effects of acute carbaryl exposure on clotting factor activity in the rat. Ecotoxicol Environ Saf. 1984; 8:280-283.
12. Kiran R, Sharma M, Bansal RC. In vivo effect of carbaryl on some enzymes of rat liver, kidney and brain. Pesticides. 1985; 19:42-43.