IJCRR - 8(11), June, 2016
Pages: 15-22
ROLE OF BIOFILMS IN STAPHYLOCOCCUS COLONISING INTRAVENOUS CATHETERS
Author: Aruna Jadhav, Vaishali Dohe, Anju Kagal, Renu Bharadwaj
Category: Healthcare
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Abstract:
Introduction: Staphylococci are major nosocomial pathogen associated with indwelling medical devices, especially with intravascular catheter (I V) related infections. Major virulence factor of Staphylococcus is its ability to form biofilm on polymeric surfaces.
Objective:
• To isolate and identify the pathogens colonising IV catheters.
• To select the Staphylococcus species and to detect their ability to produce biofilms along with antimicrobial susceptibility testing.
Material and methods: A total of 373 IV catheter tips from 373 patients were collected and processed for isolation of bacterial pathogen. Total 119(31.9%) IV catheter tips were culture positive. 100 Staphylococcus strains were tested for biofilm detection by Tissue culture plate (TCP) method and Tube method (TM). Antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method as per CLSI guidelines. The time frame since insertion and removal of catheter was also noted.
Results: 125 organisms were isolated from 119(31.9%) culture positive IV catheter tips. Staphylococcus spp.(80%) was the most common organism followed by Enterococcus spp(9% ). 93(24.4%) catheters were colonised within one week of insertion.56% of the Staphylococci were Coagulase negative staphylococci (CoNS) and 44% were S.aureus. Of 100 Staphylococcus spp. 84% were biofilm producer by TCP method and 75% by Tube method. Sensitivity and specificity of TM method vis-a-vis TCP method was 89.3%and 100% respectively.
Conclusion: Majority of Staphylococcus isolated from IV catheters were biofilm producers. TCP method is standard method for detection of strong, moderate and weak biofilm producing strains, but TM method is technically simple. Antimicrobial resistance was significantly higher in biofilm producing Staphylococcal species.
Keywords: Tissue culture plate (TCP), Tube method (TM), Biofilm, Intravascular catheter (I V).
Citation:
Aruna Jadhav, Vaishali Dohe, Anju Kagal, Renu Bharadwaj. ROLE OF BIOFILMS IN STAPHYLOCOCCUS COLONISING INTRAVENOUS CATHETERS International Journal of Current Research and Review. 8(11), June, 15-22
References:
1. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of Staphylococci: An evaluation of three different screening methods. Indian J. Med. Microbiol. 2006; 24:25-9.
2. Harald S, Bernd J, Barry M.F, Catheter-Related Infections.2nd edition, Taylor and Francis e-Library, 2006
3. Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime producing strains of staphylococcus epidermidis to smooth surfaces. Infect Immun 1982; 37(1):318-26.
4. Yazdani R, Oshaghi M, Havayi A, Pishva E, Salehi R, Sadeghizadeh M, Foroohesh H. Detection of icaAD Gene and Biofilm Formation in Staphylococcus aureus Isolates from Wound Infections. Iranian J Publ Health. 2006; 35( 2):25-28.
5. Vuong C, Voyich JM, Fischer ER, Braughton KR, Whitney AR, DeLeo FR et al Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system. Cell Microbiol.2004; 6(3):269–75.
6. Donlan RM. Biofilms: microbial life on surfaces: Emerg Infect Dis.2002; 8(9): 881-90.
7. Lawrence JR, Neu TR. Confocal Laser scanning microscopy for analysis of microbial biofilms. Meth Enzymol 1999, 310: 131- 44
8. Freeman DJ, Falkiner FR, Keane CT.New method for detecting slime production by coagulase-negative Staphylococci. J Clin Pathol 1989; 42(8): 872-74.
9. Christensen GD, Simpson WA, Younger JA, Baddour LM, Barrett FF, Melton DM et al. Adherence of cogulase negative Staphylococci to plastic tissue cultures:a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol 1985;22(6):996-06.
10. Catheter tip culture updated on 2007 (www.asm.org), laboratory service manual micro/viro. Available from: (www.childrens mn.org).
11. Bouza E, Alvarado N, Alcala L, Sa´nchez-Conde M et al. A Prospective, Randomized, and Comparative Study of 3 Different Methods for the Diagnosis of Intravascular Catheter Colonization. Clinical Infectious Diseases 2005; 40:1096–100.
12. Brun-Buisson C, Abrouk F, Legrand P, Huet Y, Larabi S, Rapin M.Diagnosis of central venous catheter–related sepsis: critical level of quantitative tip cultures. Arch Intern Med 1987; 147:873–77.
13. Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. Color Atlas and Textbook of Diagnostic Microbiology. 6th edition, San Francisco Lippincott. 2006.
14. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing; twenty two informational supplements. M100-S22; vol. 32.No.1: January 2012.
15. Stepanovi? S, Vukovi? D, Hola V, Di Bonaventura G, Djuki? S, Cirkovi? I et al .Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci. APMIS. 2007;115(8):891-99
16. Singhai M, Malik A, Shahid M, Malik MA, Goyal R. A Study on Device-Related Infections with Special Reference to Biofilm Production and Antibiotic Resistance. J Glob Infect Dis. 2012; 4(4): 193–98.
17. Rao SD, Joseph MP, Lavi R, Macaden R. infections related to vascular catheters in a pediatric intensive care unit. Indian Pediatrics 2005; 42:667-72.
18. Nahirya P, Byarugaba J, Kiguli S, Kaddu-Mulindwa D. Intravascular catheter related infections in children admitted on the paediatric wards of Mulago Hospital, Uganda. Afr Health Sci. 2008; 8(4):206-16.
19. Gahlot R, Nigam C, Kumar V, Gupta M. Catheter Related Blood Stream Infections in ICU: A study from North India. Int J Infect Control. 2013; 9:1-4.
20. Chopdekar K, Chande C, Chavan S, Veer P, Wabale V, Vishwakarma K et al. Central venous catheter-related blood stream infection rate in critical care units in a tertiary care, teaching hospital in Mumbai. Indian J Med Microbiol. 2011; 29(2):169- 71.
21. Prasad S, Nayak N, Satpathy G, Nag HL, Venkatesh P, Ramakrishnan S. Molecular and phenotypic characterization of Staphylococcus epidermidis in implant related infections. Indian J Med Res.2012; 136(3):483-90.
22. Patil HV, Patil VC, Ramteerthkar MN, Kulkarni RD. Central venous catheter-related bloodstream infections in intensive care unit. Indian J Crit Care Med. 2011;15(4): 213–23.
23. Khanna V, Mukhopadhayay C, K E V, Verma M, Dabke P. Evaluation of Central Venous Catheter Associated Blood Stream Infections: A Microbiological Observational Study. J Pathog.2013; 2013: 936864.
24. Bose S , Khodke M, Basak S , Mallick S K, et al. Detection Of Biofilm Producing Staphylococci: Need Of The Hour. Journal of Clinical and Diagnostic Research. 2009; (3):1915-20.
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