IJCRR - 8(13), July, 2016
Pages: 06-11
EFFECT OF DIFFERENT PROCESSING METHODS ON POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF BROAD BEANS (VICIA FABA)
Author: Pinki Saini, Priyanka Singh, Shreyasi Dubey, and Ayushi Srivastava
Category: Healthcare
[Download PDF]
Abstract:
Objective: The effects of processing on total phenolic components and antioxidant activity in commonly consumed broad bean was investigated. Methods: The raw and processed samples were extracted with 70% methanol and analysed for antioxidant components and antioxidant activity.
Results: Processing of legumes caused decrease in total phenolic content when compared to the raw samples. However, the dry heating caused remarkable increase in tannin contents (6.98±0.53 g TAE/100 g extract). The flavanoid and β carotene content was significantly reduced on processing of samples. Raw sample of D. lablab was found to possess the highest DPPH (73.5±2.5%), Reducing power (4.9±0.68 mg ascorbic acid/gm) and Iron chelating capacity than other samples. Conclusion: Maximum retention of antioxidant activity was observed in dry heated samples. Higher correlation was found between phenolic content and chelating capacity (r2=0.945) but a poor correlation with DPPH. Moreover, the content of tannins gave good correlation (r2=0.745–0.913) with Iron chelating and DPPH assays.
Keywords: Processing, Antioxidant, Broad beans, Total phenolics, Correlation
Citation:
Pinki Saini, Priyanka Singh, Shreyasi Dubey, and Ayushi Srivastava. EFFECT OF DIFFERENT PROCESSING METHODS ON POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF BROAD BEANS (VICIA FABA) International Journal of Current Research and Review. 8(13), July, 06-11
References:
1. A. Djeridane, M. Yousfi, B. Nadjemi, D. Boutassouna, P. Stocker, N. Vidal, Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds, Food Chem. 97 (2006) 654–660.
2. A.O.A.C. Official methods of analysis, Association of Official Analytical Chemists International. Maryland, USA, 2005.
3. B.D. Oomah, A. Cardador-Martinez, G. Loarca-Piña, Phenolics and antioxidative activities in common beans (Phaseolus vulgaris L), J Sci Food Agric, 85 (2005) 935–942.
4. B.J. Xu, S.H. Yuan, S.K.C. Chang, Comparative analyses of phenolic composition, antioxidant capacity, and color of cool season legumes and other selected food legumes, J Food Sci .72(2007)167–177.
5. C. M. Ying, A. Azlan, S. Hasan Al-Sheraji, F. A. Hassan and K. N. Prasad, Antioxidant Activities and Total Phenolic Content in Germinated and Non-Germinated Legume Extracts Following Alkaline-Acid Hydrolysis, Pak J Nutr, 12 (2013)1036-1041.
6. C.F. Chau, P.C.K. Cheung, Y.S. Wong, Hypocholesterolemic effects of protein concentrate from three Chinese indigenous legume seeds, J Agric Food Chem., 46(1998)3698–3701.
7. D. Kim, O. Chun, Y. Kim, H. Moon and C. Lee, Quantification of phenolics and their antioxidant capacity in fresh plums, J. Agric. Food Chem., 51(2003) 6509-6515
8. E.A. Komolafe and V.S. Obayanju, Principle of Food Processing and Preservation. 1st Edn., Double Birth Publishers, USA., (2003) 87.
9. F. Yamaguchi, T. Ariga, Y. Yoshirmura, K. Nakazaw, Antioxidative and antiglycation activity of garcinol from Garcinia indica fruit rind, J Agric Food Chem., 48 (2000) 180–185.
10. F.C. Barroga, A.C. Laurena, E.M.T. Mendosa, Polyphenols in mung bean (Vigna radiata (L.) Wilczek): determination and removal. J Agri and Food Chem, 33 (1985) 1006-1009
11. G. Yaciuk, and J. Sofose, Food drying proceeding of a not shop held at Edmonton Albeta, 6th-9th July (1981)
12. H. H.F. Koolen, da Silva M.A. Felipe, F. C. Gozzo, A. Q.L. de Souza, A. D.L. de Souza. 2013, Antioxidant, antimicrobial activities and characterization of phenolic compounds from buriti (Mauritia flexuosa L. f.) by UPLC–ESI-MS/MS, Food Res Int, 51 (2013) 467–473.
13. K. Shimada, K. Fujikawa, N.T. Yahara, Antioxidative properties of xanthin on autoxidation of soybeanoil in cyclodextrin emulsion, J Agric Food Chem., 40(1992) 945–948.
14. M. Carbonaro, F.Virgili, E. Carnovale, Evidence for protein tannin interaction in legumes: Implications in the antioxidant properties of faba bean tannins, LWT Food Sci Technol., 29(1996)743–750.
15. M. Oyaizu, Studies on products of browning reaction: antioxidative activity of products of browning reaction prepared from glucosamine, Japanese J Nutr. 44 (1986) 307–315.
16. N.E. Rocha-Guzman, R.F. Gonzalez-Laredo, F.J. Ibarra-Perez, C.A. Nava- Berumen, J.F. Gallegos-Infante, Effect of pressure cooking on the antioxidant activity of extracts from three common bean (Phaseolus vulgaris L.) cultivars, Food Chem. 10(2007) 31–35.
17. O.L. Milo, Nutraceuticals and functional foods. Food Technology, 58 (2004) 65–68.
18. P. Saini and P. Singh, Antioxidant activity and antimicrobial property of some Indian Spices, Trends in Biosci, 8(19) (2015) 5261-5267.
19. P. Siddhuraju and K. Becker, Studies on antioxidant activities of mucuna seed (Mucunapruriens var. utilis) extracts and certain non-protein amino/ imino acids through in vitro models, J Sci Food Agric., 83 (2003) 1517–1524.
20. P. Siddhuraju, The antioxidant of phenolic compounds extracted from defatted raw and dry heated Tamarindus indica seed coat. Lebensmittel Wis- senschaft und Technologie, 40 (2007) 982-90
21. P. Siddhuraju, V. Maheshu, N. Loganayaki, S. Manian, Antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from processed indigenous legumes, Macrotyloma uniflorum (Lam.) Verdc. and Dolichos lablab, L. Food. 2 (2008) 159–167.
22. P.J. Tsai, C.H. She, Significance of phenol-protein interactions in modifying the antioxidant capacity of peas, J Agric Food Chem. 54 (2006)8491–8494.
23. R. Amarowicz, M. Karamac, F. Shahidi, Antioxidant activity of phenolic fractions of lentils (Lens culinaris), J Food Lipids, 10(2003)1–10.
24. R. Fernandez-Orozco, H. Zielinski, M.K. Pisku?a, Contribution of low-molecular-weight antioxidants to the antioxidant capacity of raw and processed lentil seeds, Nahrung Food. 47(2003) 291–299.
25. S. Ranganna. Handbook of Analysis and Quality Control for Fruit and Vegetable Products, Tata McGraw-Hill Education, 2005.
26. S.K. Yadav, A. Sehgal, Effect of home processing on ascorbic acid and beta-carotene content of spinach (Spinachia oleracia) and amaranth (Amaranthus tricolor) leaves, Plant Foods for Human Nutrition. 47 (1995) 125–131.
27. S.K. Yadav, A. Sehgal, Effect of home processing on ascorbic acid and beta-carotene content of bathua (Chenopodium album) and fenugreek (Trigonella foenumgraecum) leaves, Plant Foods for Human Nutr,50 (1997) 239–247.
28. U. Krings and R.G. Berger. Antioxidant activity of some roasted foods, Food Chem, 72 (2001) 223-229.
29. V. Maheshu, D.T. Priyadarsini, D. Teepica and J.M. Sasikumar, Effects of processing conditions on the stability of polyphenolic contents and antioxidant capacity of Dolichos lablab L, J Food Sci Technol., 50(4) (2013) 731–738.
30. V. Pascharicha, G. Satpathy, R. K. Gupta, Phytochemical and Antioxidant activity of underutilized legume Vicia faba seeds and formulation of its fortified biscuits, J Pharma and Phytochem, 3 (2014) 75-80.
31. W. Brand-Williams, M.E. Cuvelier, C. Berset, Use of a free radical method to evaluate antioxidant activity, LWT - Food Sci and Technol 28(1995) 25-30.
32. W.J. Yen, L.W. Chang, P.D Duh, Antioxidant activity of peanut seed testa and its antioxidative component, ethyl protocatechuate, LWT Food Sci Technol, 38 (2005) 193–200.
33. X.Y. Ye, H.X. Wang, T.B.G, Dolichin, a new chitinase-like antifungal protein isolated from field beans (Dolichos lablab), Biochem Biophys Res Commun., 269 (2000) 155–159.
34. Y. S. Velioglu, G. Mazza , L. Gao, B. D. Oomah, Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products, Journal of Agricultural and Food Chemistry. 46 (1998) 4113–4117.


|