IJCRR - 9(6), March, 2017
Pages: 25-29
Consumption of GSH with the Increase in Oxidative Stress in Chronic Obstructive Pulmonary Disease (COPD) Patients
Author: Rupali S. Pawar, Subodhini A. Abhang
Category: Healthcare
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Abstract:
Introduction: Oxidant-antioxidant imbalance plays a major role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Recently, most of the studies are concentrated on the evaluation of oxidant-antioxidant balance in exhaled breath condensate and in other body fluids in COPD patients.
Aims and Objectives: In this study, we investigated oxidant-antioxidant balance in systemic circulation of patients with COPD. Serum malondiladehyde (MDA), protein carbonyl (PC) and whole blood reduced glutathione (GSH) levels were determined in 60 patients with stable COPD and it compared with 60 age and sex matched healthy controls.
Materials and Methods: The levels of serum MDA, protein carbonyl and whole blood reduced glutathione were determined according to Buge and Aust method, Levin et al and Beutler et al method.
Results: Serum MDA and PC levels were significantly increase in COPD patients as compared to healthy controls. We found significantly decreased levels of erythrocyte GSH in stable COPD patients as compared to healthy controls.
Conclusion: Tobacco smoking cause increase in oxidative stress and reduction in antioxidant level in COPD patients. From these findings we conclude that there is disturb oxidant – antioxidant balance in COPD patients and this imbalance is related to long term history of tobacco smoking.
Keywords: COPD, Reduced glutathione, Malondialdehyde, Protein carbonyl
Citation:
Rupali S. Pawar, Subodhini A. Abhang. Consumption of GSH with the Increase in Oxidative Stress in Chronic Obstructive Pulmonary Disease (COPD) Patients International Journal of Current Research and Review. 9(6), March, 25-29
References:
1. WHO site http:// www.who.int world bank/ WHO global burden of disease study.
2. MacNee W. Pulmonary and systemic oxidant/antioxidant imbalance in chronic obstructive pulmonary disease. Proc Am Torac Soc. 2005; 2:50-60
3. Pryor WA, Prier DG, Church DF. Electrons spin resonance study of main-stream and side stream smoke: nature of free radicals in gas-phase smoke and in cigarette tar. Environ Health Perspect 1983; 47:345-55
4. Kumar V, Abbas A, Fausto N, Aster J. The Lung. In: Robbins and Cotran pathologic basis of disease. 8th edin, Elsevier, New Delhi 2010; 683-92
5. Devasagayam TPA, Boloor KK, Ramsarma T. Methods for estimatinf lipid peroxidation: Analysis of merits and demerits (minireview). Indian J Bioche Biophys 2003; 40: 300-308
6. Malondialdehyde from Wkipedia, the free encyclopaedia (serial online) cited 27th Aug. 2010. Available from :http//en.wikipedia.org/wiki/malondialdehyde
7. Lopaczynski W, Zeisel SH. Antioxidants, programmed cell death, and cancer. Nutr Res. 2001;21:295–307.
8. Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl group as biomarkers of oxidative stress. Clin Chim Acta 2003;329(1-2):23-38
9. Rai RR, Phadke MS. Plasma oxidant-antioxidant status in different respiratory disorders. Indian J Clin Biochem 2006; 21(2): 161-64
10. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8): 118-126
11. Antonicelli F, Parmentier M, Drost EM, Hirani N, Rahman I, Donaldson K, MacNee W. Nacystelyn inhibits hydrogen peroxide mediated interleukin-8 expression in human alveolar epithelial cells. Free Rad Biol Med. 2002;32:492–502.
12. Aoki T, Suzuki Y, Suzuki K, Miyata A, Oyamada Y, Takasugi T, Mori M, Fujita H, Yamaguchi K. Modulation of ICAM-1 expression by extra-cellular glutathione in hyperoxia-exposed human pulmonary artery endothelial cells. Am J Respir Cell Mol Biol. 1996;15: 319–327.
13. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J.Lab.Clin. Med.1963; 61(5): 882-888.
14. Buege JA, Aust SD. Microsomal lipid peroxidation. Method Enzymol. 1978; 52:302-310.
15. Levin RL, Garland D, Oliver CN, Amici A, Climet I, Lenz AG. Determination of carbonyl content in oxidatively modified proteins. Meth. Enzymol. 1990;186: 464-78.
16. Bradfoed MM. A rapid and sensitive method for the quantitation of microgram of
protein utilizing the principle of protein dye binding. Analy. Bioch. 1976;72:248-54.
17. Pawar RS, Abhang SA, Borale P, Lokhande R. Study of correlation of pulmonary function test with the markers of oxidative stress and non-enzymatic antioxidants in chronic obstructive pulmonary disease (COPD) patients. British Journal of Medicine and Medical Research 2014; 4(28):4710-4722.
18. Parija M, Bobby Z, Kumar VS, Saradha B. Oxidative stress and protein glycation in patients with chronic obstructive pulmonary disease. Indian J Physiol Pharmacol. 2005; 49(1):95-98
19. Calikoglu M, Unlu A, Tamer L, Ercan B, Bugdayci R, Atik U. The levels of serum vitamin C, malondialdehyde and erythrocyte reduced glutathione in chronic obstructive pulmonary disease and in healthy smokers. Clin Chem Lab Med 2002;40(10): 1028-31
20. Toorn MV, Maria P, Varies S, Slebos D, Bruin HG, Abello N et al. Cigarette smoke irreversibly modifies glutathione in airway epithelial cells. Am J Physiol 2007; 293:1156-62
21. Ozbay B, Dulger H. Lipid peroxidation and antioxidant enzymes in Turkish population: Relation to age, gender, exercise and smoking. Tohoku J Exp Med 2002;197:119-24
22. Yessica DT, Maria LG, Ivonne MO, Hicks JJ. Correlation of plasma protein carbonyl and C-reactive protein with GOLD stage progression in COPD patients. Open Respir Med J 2009; 3: 61-66
23. Daga MK , Chhabra R, Sharma B, Mishra TK. Effects of exogenous vitamin E supplementation on the levels of oxidants and antioxidants in chronic obstructive pulmonary disease. J Biosci. 2003; 28(1): 7-11
24. Kirkil G, Muz MH, Seckin D, Sahin K, Kucuk O. Antioxidant effect of zinc picolinate in patients with chronic obstructive pulmonary disease. Respir Med 2008;102:840-44
25. Pryor WA, Prier DG, Church DF. Electron-spin resonance study of main-stream and sidestream cigarette smoke: nature of the free radicals in gas-phase smoke and in cigarette tar. Environ Health Prospect 1983;47:345-55
26. Henneke JH, Leo MAH, Geraedts MCP, Hafmans T, Josw RV, Richard D. et al. Oxidative and nitrosative stress in diaphragm of patients with COPD. Intr. J. COPD.2006;1(2):173-179.
27. Warren JS, Johnson KJ, Ward PA. Consequence of oxidant injury. In: Crystal RG, Weibel ER, Barnes PJ eds. The Lung: Scientific foundation. New York N.Y. Raves Press Ltd. 1997; 2279-2288
28. Mesia-Vela S, Yeh CC, Austin JHM, Dounel M, Powell CA, Reeves A, Santella RM, Stevenson L, Yankelevitz D, Barr GR. Plasma carbonyls do not correlate with lung function or computed tomography measures of lung density in older smokers. Biomarkers 2008; 13(4): 422-434
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