IN VITRO EVALUATION OF ANTIMICROBIAL EFFECT OF FRESH GARLIC EXTRACT AND ITS INTERACTION WITH CONVENTIONAL ANTIMICROBIALS AGAINST ESCHERICHIA COLI ISOLATES

Vishal Gaekwad; N.A. Trivedi

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IJCRR - 5(1), January, 2013

Pages: 106-114

IN VITRO EVALUATION OF ANTIMICROBIAL EFFECT OF FRESH GARLIC EXTRACT AND ITS INTERACTION WITH CONVENTIONAL ANTIMICROBIALS AGAINST ESCHERICHIA COLI ISOLATES

Author: Vishal Gaekwad, N.A. Trivedi

Category: Healthcare

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Abstract:

Escherichia coli (E. coli) is the most common etiological agent, for causation of uncomplicated urinary tract infection. At the same time it is one of the bacterial strains account for wide range of antimicrobial resistance. Garlic (allium sativum) possesses antimicrobial activity against wide variety of organisms. In the present study antimicrobial activity of fresh garlic extract (FGE) was assessed by well diffusion as well as tube dilution method and effects of combination of ampicillin and ciprofloxacin with FGE were studied by well diffusion method against the clinical isolates of E Coli spp. FGE per se produced dose dependent increase in zone of inhibition. In the combination study, addition of FGE with various concentrations of ampicillin (2.5-20mcg/ml) resulted in marked further increase in zone of inhibition than that produced by ampicillin per se at the respective concentration. While combining different concentrations of ciprofloxacin with FGE, ciprofloxacin 1.25mcg/ml with 20mg/ml and 40mg/ml of FGE produced synergistic effect. This study suggests possibility of concurrent use of antimicrobial drugs and FGE in treating infections caused E. coli spp. Further detailed pharmacokinetic and in vivo studies of garlic are required.

Keywords: Garlic; Ampicillin; Ciprofloxacin; Antimicrobial activity; E Coli

Citation:

Vishal Gaekwad, N.A. Trivedi. IN VITRO EVALUATION OF ANTIMICROBIAL EFFECT OF FRESH GARLIC EXTRACT AND ITS INTERACTION WITH CONVENTIONAL ANTIMICROBIALS AGAINST ESCHERICHIA COLI ISOLATES International Journal of Current Research and Review. 5(1), January, 106-114

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