IJCRR - Vol 05 Issue 01, January, 2013
INDUCIBLE CLINDAMYCIN RESISTANCE AMONG CLINICAL ISOLATES OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
Author: Nilima R. Patil, Ulhas S. Mali, Sunanda A. Kulkarni, M. V. Ghorpade, Poorva P. Bhave (Sule)
The resistance to antimicrobial agents among Methicillin resistant Staphylococcus aureus (MRSA) is an increasing problem. Clindamycin is commonly used for the treatment of serious soft tissue infections produced by Staphylococcus aureus and treatment failures are reported during therapy. Routine antibiotic sensitivity test for Clindamycin susceptibility may fail to identify iMLSB (inducible MLSB) strains of Staphylococcus aureus due to erm gene. Present study was carried out to find out the percentage of inducible Clindamycin resistance in MRSA in our hospital using D-test. 250 isolates of Staphylococcus aureus from various clinical samples were confirmed by standard protocol and included in this study. MRSA were detected by using Oxacillin (1ug) and Cephoxitin (30ug) disc diffusion method. Results were interpreted according to CLSI criteria. Antibiotic sensitivity to routine antimicrobial agents was carried out using Kirby Bauers disc diffusion method. D-test was performed on all Erythromycin resistant Staphylococcus aureus to detect inducible Clindamycin resistance and constitutive Clindamycin resistance. Out of 250 isolates of Staphylococcus aureus, 90(36%) were found to be MRSA. Among these, 46(51.11%) were Erythromycin resistant. 17(36.95%) isolates showed inducible Clindamycin resistance, 4(8.69%) showed constitutive Clindamycin resistance and 25(54.34%) showed MS phenotype in MRSA. Conclusion – We therefore conclude that it is necessary to perform D- test for detection of inducible Clindamycin resistance among Staphylococcus aureus in routine antibiotic sensitivity test. Therapeutic failures may thus be avoided.
Keywords: Staphylococcus aureus, inducible MLSB, constitutive Clindamycin resistance, D-test, MRSA.
Nilima R. Patil, Ulhas S. Mali, Sunanda A. Kulkarni, M. V. Ghorpade, Poorva P. Bhave (Sule). INDUCIBLE CLINDAMYCIN RESISTANCE AMONG CLINICAL ISOLATES OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS International Journal of Current Research and Review. Vol 05 Issue 01, January, 44-48
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