IJCRR - 5(2), January, 2013
INDUCTION OF A CALCIUM DEFICIENCY IN CUTTINGS OF GRAPE VINE (Vitis vinifera L.)
Author: Bechir Ezzili
Category: General Sciences
Rooted cuttings of grape vine, cvs. ‘Black Grenache’, ‘Carignane’ and ‘Alicante Bouschet’, kept at 25 ± 2° C in the dark, I transferred it 30-days after budburst to a greenhouse at 25°C (day), 20°C (night) and 12 hr light. A week after, foliar growth started and normal green pigmentation appeared. 21 days later, the synthesis of anthocyanins appeared in the leaves and stems. After 28 days, calcium content, expressed as the percentage of dry matter in the shoot apex, decreased and symptoms of calcium deficiency appeared. A hypothesis was proposed to explain the mechanism inducing calcium deficiency by the pretreatment to darkness.
Keywords: Cutting one eye, pretreatment by darkness, calcium deficiency.
Bechir Ezzili. INDUCTION OF A CALCIUM DEFICIENCY IN CUTTINGS OF GRAPE VINE (Vitis vinifera L.) International Journal of Current Research and Review. 5(2), January, 28-37
1. Barta, D. J., Tibbits, T. W. (2000). Calcium localization and Tipp burn development in lettuce leaves during early enlargement. J. Am. Soc. Hort. Sci. 125: 294-298.
2. Briggs, W.R., Mosinger, E., Schafer, E. (1988). Phytochrom regulation of greening in barley- Effets on chlorophyll accumulation. Plant Physiol. 86: 435-440.
3. Bruzau, F., Juste, C. et Poujet, R. (1968). Mise en évidence d'une carence en calcium chez la vigne cultivée en solution nutritive. C.R.A.S.S. PARIS. 266 : 116-118.
4. Dekock, P.C, Dyson, P.W, Hall, A. et Grabowska, F. (1975). Metabolic changes associated with calcium deficiency in potato sprouts. Potato Research. 18: 573-581.
5. Drazeta, L. A., Hall A. J. Volz, R., Jameson, P.E. (2004). Causes and effect of change in xylem functionality in apple fruit. Ann. Bot. 93: 275-282.
6. Epstein, E. (1973). Flow in the phloem and the immobility of calcium and boron : a new hypothesis in support of an old one. Experimentia. 29: 133-134.
7. Ezzili, B., Habib, J., Darne, G., et Chemli, R. (1997). lnfluence de la date de prélèvement sur les teneurs en anthocyanes et en éléments minéraux du cépage Alicante Bouschet cultivé à El khenguet (Tunisie); Bull. OIV. 70: 795-796.
8. Ezzili, B; Darne, G. et Bejaoui, M. (1999). Mise au point d’une technique de production d’anthocyanes dans les feuilles de boutures à un œil de Carignan (Vitis vinifera L.) cultivées au laboratoire. J. Int. Sci. Vigne. Vin. 33: 9-18.
9. Ferguson, I.B. et Bollard, E. G. (1976). The movement of calcium in woody stems. Ann. Bot. 40: 1057-1065.
10. Hocking, P.G., Pate, J.S., .Atkins, A. et Sharkey, P.J. (1978). Diurnal patterns of transport and accumulation of minerals in fruiting plants of Lupinus augustifolius. Ann. Bot. 42 : 1277-1290.
11. Glad, O., Regnard, J.L., Queron, Y., Brun, O., Morot-Gaudry, J.F. (1992). Flux and chemical composition of xylem exsudates from Chardonnay grapevine: Temporal evolution and effect of recut. Am. J. Enol. Vitic. 43: 275-282.
12. Kirkby, E.A., Pilbeam, D.J. (1984). Calcium as nutrient. Plant cell and environment, 7: 397-405.
13. Marschner, H. (1995). Mineral Nutrition of Higher Plants. Academic Press, Amsterdam, 2 ed edition
14. Palzkill, D.A., Tibbits, T.W., Williams, P.H. (1976). Enhancement of calcium transport to inner leaves of Cabbage for prevention of Tipburn. J. Amer. Soc. Hort. Sci., 101: 605- 648.
15. Palzkill, D. A., Tibbits T. W., (1977). Evidence that root pressure is required for calcium transport to head leaves of Cabbage. Plant Physiol.
16: 682-694. 16. Raven, A. (1977). H+ and Ca++ in phloem and symplast : Relation of relative immobility of the ions to the cytoplasmic nature of transport paths. New Phytol. 79: 465-480.
17. Saure, M.C. (1996). Reassessment of the role of calcium in development of bitter pit in apple .Aust. J. Plant. Physiol. 23: 237-243.
18. Saure, M.C. (2005). Calcium translocation to fleshy fruit: its mechanism and endogenous control. Scientia Horticulturae 105: 65-89.
19. Shear, C. B. (1975). Calcium related disorders of fruits and vegetables. Hort Science, 10:361-365.
20. Van De Geijn S. C. Smeulders E. (1981). Diurnal changes in the flux of calcium toward meristems and transpiring leaves in Tomato and Maize plants. Planta, 151: 265- 271.
Legende of figures
Procedure for the induction of a calcic deficiency at the laboratory
1 Bud is plastered in order to induce roots.
2 When there are at least 3 roots the bud is removed the plaster.
3 bud bursting in darkness
4 Stage six very reduced sheets stage 30 days in darkness
5 Stage 30 days in darkness and in greenhouse. On the left, shoots in total darkness can have 7, 8, 9, 12 very reduced a pale yellow sheets. On the right, cuttings are cultivated in greenhouse give normal axes with spread out and rather large sheets. To note here the difference of lengthening of the branches and growth of the appreciable sheets.
6 Cutting transferred from the darkness to the greenhouse during 15 days. Note here the beginning of colouring of the stems.
7 Cutting transferred from the darkness to the greenhouse during 22 days. To note Curve of the stem of approximately 45°C and sunk red colouring of the carrying branch.
8 Cutting transferred from the darkness to the greenhouse during 30 days. To note the curve of an angle equal to 135°C and beginning of the drying of the apex which starts approximately at a point located at the third of the branch.