IJCRR - 5(2), January, 2013
Pages: 28-37
INDUCTION OF A CALCIUM DEFICIENCY IN CUTTINGS OF GRAPE VINE (Vitis vinifera L.)
Author: Bechir Ezzili
Category: General Sciences
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Abstract:
Rooted cuttings of grape vine, cvs. ‘Black Grenache’, ‘Carignane’ and ‘Alicante Bouschet’, kept at 25 ± 2° C in the dark, I transferred it 30-days after budburst to a greenhouse at 25°C (day), 20°C (night) and 12 hr light. A week after, foliar growth started and normal green pigmentation appeared. 21 days later, the synthesis of anthocyanins appeared in the leaves and stems. After 28 days, calcium content, expressed as the percentage of dry matter in the shoot apex, decreased and symptoms of calcium deficiency appeared. A hypothesis was proposed to explain the mechanism inducing calcium deficiency by the pretreatment to darkness.
Keywords: Cutting one eye, pretreatment by darkness, calcium deficiency.
Citation:
Bechir Ezzili. INDUCTION OF A CALCIUM DEFICIENCY IN CUTTINGS OF GRAPE VINE (Vitis vinifera L.) International Journal of Current Research and Review. 5(2), January, 28-37
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Legende of figures
Procedure for the induction of a calcic deficiency at the laboratory
1 Bud is plastered in order to induce roots.
2 When there are at least 3 roots the bud is removed the plaster.
3 bud bursting in darkness
4 Stage six very reduced sheets stage 30 days in darkness
5 Stage 30 days in darkness and in greenhouse. On the left, shoots in total darkness can have 7, 8, 9, 12 very reduced a pale yellow sheets. On the right, cuttings are cultivated in greenhouse give normal axes with spread out and rather large sheets. To note here the difference of lengthening of the branches and growth of the appreciable sheets.
6 Cutting transferred from the darkness to the greenhouse during 15 days. Note here the beginning of colouring of the stems.
7 Cutting transferred from the darkness to the greenhouse during 22 days. To note Curve of the stem of approximately 45°C and sunk red colouring of the carrying branch.
8 Cutting transferred from the darkness to the greenhouse during 30 days. To note the curve of an angle equal to 135°C and beginning of the drying of the apex which starts approximately at a point located at the third of the branch.





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