IJCRR - Vol 05 Issue 07, April, 2013
INVESTIGATION OF ANTI BACTERIAL AND ANTI FUGAL POTENTIALS OF MACARANGA PELTATA
Author: Meenakshi Verma, Narayanan K., Mitali Thakar B., Subrahmanyam V.M., Venkata Rao J., Dhanaraj S.A., Vasanth Raj P.
Category: General Sciences
Macaranga peltata belonging to Euphorbiaceae family. Leaves and stem bark were separated, shade dried and extracted separately using methanol. The concentrated methanolic extracts of Macaranga peltata were subjected to antimicrobial studies. The percentage yield of the leaves and stem bark were found to be 47% and 30% respectively. The anti-bacterial activity of Leaf and Stem Bark extracts of Macaranga peltata was determined by Cup-plate method and Zone of Inhibition was measured. Zone of Inhibition was found to be 16mm for Leaf extract and 13mm and 14mm for Stem Bark extract in two different sets against Escherichia coli. Out of two Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus Zone of Inhibition was obtained only for Staphylococcus aureus that was 16mm for Leaf extract and 12mm and 13mm for Stem Bark. Leaf extracts showed better anti-bacterial activity than the Stem Bark extract against both Gram-positive and gram-negative bacteria. Minimum Inhibitory concentration (MIC) for Leaf extract was between 62.5μg/ml to 125μg/ml for Escherichia coli, 125μg/ml to 250μg/ml for Pseudomonas aeruginosa, 62.5μg/ml to 125μg/ml for Bacillus subtilis and 31.25μg/ml to 62.5μg/ml for Staphylococcus aureus. Whereas MIC for Stem Bark extract was between 500μg/ml to 1000μg/ml for Escherichia coli, 250μg/ml to 500μg/ml for Pseudomonas aeruginosa, 62.5μg/ml to 125μg/ml for Bacillus subtilis and 62.5μg/ml to 125μg/ml for Staphylococcus aureus. Ciprofloxacin used as standard antibiotic showed MIC between 1.953 μg/ml to7.813 μg/ml for all four bacterial strains.
Keywords: Macaranga peltata, Euphorbiaceae, anti-bacterial and Cup-plate method
Meenakshi Verma, Narayanan K., Mitali Thakar B., Subrahmanyam V.M., Venkata Rao J., Dhanaraj S.A., Vasanth Raj P.. INVESTIGATION OF ANTI BACTERIAL AND ANTI FUGAL POTENTIALS OF MACARANGA PELTATA International Journal of Current Research and Review. Vol 05 Issue 07, April, 17-24
1. Mahesh B. and. Satish S.; Antimicrobial Activity of Some Important Medicinal Plant against Plant and Human Pathogens; World Journal of Agricultural Sciences; 2008; 4 (S): 839-843.
2. Duraipandiyan V.,Ayyanar M.and Ignacimuthu S.; Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India; BMC Complementary and Alternative Medicine 2006, 6:35
3. Lino A., Deogracious O.; The in-vitro antibacterial activity of Annona senegalensis , Securidacca longipendiculata and Steganotaenia araliacea - Ugandan medicinal plants; African Health Sciences; 2006; 6(1): 31-35
4. Uniyal S.K., Singh K.N., Jamwal P. and Lal B.; Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya; Journal of Ethnobiology and Ethnomedicine; 2006; 2:14
5. Yoder B. J., Cao S., Norris A., Miller J. S., Ratovoson F., Razafitsalama J., Andriantsiferana R., Rasamison V., and Kingston D; Cytotoxic Prenylated Stilbenes and Flavonoids from Macaranga alnifolia from the Madagascar Rainforest; Journal of Natural Products. 2007 March; 70(3): 342– 346
6. Srinivasan D., Sangeetha N., Suresh T and Perumalswamy PL. Antimicroabial activity of certain Indian medicinal plants used in folkloric medicine. Journal of Ethano Pharmacology. 2001; 74 (3): 217- 220.
7. Khandelwal, K. R. Practical Pharmacognosy, Techniques and Experiments, 8th Ed., Nirali Prakashan. 2001, pp. 45-69.
8. Pao-Chuan H., Jeng-Leun M., Shu-Hui H. Antimicrobial effect of various combinations of plant exracts. Food Microbiology 2001; 18 (1); 35- 43.
9. Laura LZ. Spices and Herbs: Their Antimicrobial activity and its determination. Journal of Food Safety 1988; 9 (2); 97-118.
10. Jennifer MA. Determination of minimum inhibitory concentrations. Journal of antimicrobial Chemotherapy 2001; 48 (suppl 1); 5-16.