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IJCRR - 5(11), June, 2013

Pages: 80-86

A COMPARATIVE EVALUATION OF HBA1C MEASUREMENT IN DIFFERENT ANTICOAGULANT VIALS AND ITS STABILITY ON STORAGE

Author: Devajit Sarmah, Booloo Sharma

Category: Healthcare

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Abstract:

Introduction: India has an overwhelming diabetic population and the importance of HbA1c testing is felt. However the need of an additional EDTA sample and due to lack of standardised laboratories in most part of India, samples are stored and transported for testing. The study aims to check the variation in HbA1c measurement in K3EDTA, Na-citrate, lithium-heparin and Na-fluoride/Na2 EDTA anticoagulant vials and check for its stability when sample are stored at 2 – 8°C for 7 days. Methods and materials: HbA1c was measured by cation exchange HPLC based BioRad D - 10 analyser after collection of sample in K3EDTA, Na-citrate, lithium-heparin and Na-fluoride/Na2 EDTA from 25 diabetic and 25 non - diabetic subjects on day 1 and on day 3, day 5 and day 7 of sample collection, after storage at 2 – 8°C. Result: There were no variation in the measured value of HbA1c in these anticoagulant vials (CV = 0.0 – 0.8%). There were no significant differences in the mean values of the HbA1c measured on day 1 and day 7 (8.2 ± 1.16 vs. 8.2 ± 1.18, P = 0.962 in diabetic and 5.3 ± 0.25 vs. 5.3 ± 0.24, P = 0.955 in non - diabetic). There were significant correlations between the HbA1c values measured on day 1 and after 7 days storage (r = 0.991 in diabetic and r = 0.957 in non –diabetic). Conclusion: HbA1c values in fresh and stored whole blood sample does not change when analysed in K3EDTA, Na-citrate, lithium-heparin and Na-fluoride/Na2 EDTA anticoagulant vials.

Keywords: HbA1c, K3EDTA, Na-citrate, lithium-heparin and Na-fluoride/Na2 EDTA, prolonged storage.

Citation:

Devajit Sarmah, Booloo Sharma. A COMPARATIVE EVALUATION OF HBA1C MEASUREMENT IN DIFFERENT ANTICOAGULANT VIALS AND ITS STABILITY ON STORAGE International Journal of Current Research and Review. 5(11), June, 80-86

References:

1. International diabetic federation, IDF Diabetic atlas, 2012, 5th edition. Available at: www.idf.org . Accessed December 20th 2012.

2. Mohan V, Sandeep S, Deepa R, Shah B, Varghese C. Epidemiology of type 2 diabetes: Indian Scenario. Indian Journal of Medical Research 2007; 125: 217-30.

3. Mohan V, Venkatraman JV, Pradeepa R. Epidemiology of cardiovascular disease in type 2 diabetes: The Indian scenario. J Diabetes Sci Technol 2010; 4:58–70.

4. The Diabetes Control and Complications Trial Research Group. The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. N Eng J Med 1993; 329(14):977- 86.

5. Diabetes Mellitus : Report of a WHO study Group, Technical Report Series 727, Geneva, World Health Organisation, 1985.

6. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2012; 35 (Suppl 1):S64–S71.

7. Sacks DB, Bruns DE, Goldstein DE, Maclaren NK, McDonald JM, Parrott M.Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Clin Chem 2002; 48:436–472.

8. Little RR, Rohlfing CL, Tennill AL, Connolly S, Hanson S. Effects of sample storage conditions on glycated hemoglobin measurement: evaluation of five different high performance liquid chromatography methods. Diabetes Technol Ther 2007; 9:36– 42.

9. Shashank R Joshi, AK Das, VJ Vijay, V Mohan Challenges in Diabetes Care in India: Sheer Numbers, Lack of Awareness and Inadequate Control. JAPI, 2008; 56:443-450.

10. Joshi SR, Das AK, Vijay VJ, Mohan V. Challenges in diabetes care in India: sheer numbers, lack of awareness and inadequate control. J Assoc Physicians India 2008; 56: 443-50.

11. Mohan V, Venkatraman JV, Pradeepa R. Epidemiology of cardiovascular disease in type 2 diabetes: The Indian scenario. J Diabetes Sci Technol 2010; 4:58–70.

12. Raheja BS, Kapur A, Bhoraskar A, Sathe SR, Jorgensen LN, Moorthi SR, et al. Diab Care Asia-India Study: diabetes care in India - current status. J Assoc Physicians India 2001; 49: 717-22.

13. Sarmah D, Sharma B. Importance and Status of HBA1C in T2DM and its Indian Perspective. Asian Journal of Biomedical and Pharmaceutical Sciences 2012; 2 (12), 1-10.

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