IJCRR - 5(11), June, 2013
A STUDY OF BLOOD CULTURE ISOLATES AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN IN A TERTIARY CARE HOSPITAL OF JAIPUR, RAJASTHAN
Author: Ashina single, Sweta Gupta, Dinesh Jain, Pushpa Durlabhji
Background: Blood culture is a gold standard for accurate detection of etiological agents of infectious diseases and can assist in choice of appropriate antimicrobial therapy. So, the present study was undertaken to identify the blood culture isolates and to study the antimicrobial susceptibility pattern of blood culture isolates. Methods: Of 500 blood specimens processed from various wards and intensive care units of Mahatma Gandhi Medical College and hospitals, Jaipur, bacteria were identified and isolated. Antibiogram was performed on all positive samples. Results: 19.6% blood culture positive cases out of which 62.2% Gram positive bacteria and 37.8% Gram negative bacteria . Among Gram positive organisms, Staph aureus was found to be the predominant isolate (47.54%) followed by Coagulase negative staphylococci (CONS) (24.5%) ,Streptococcus species (19.6%) and Enterococcus species (8.19%).Methicillin resistance was found in 34.4% strains of Staph aureus.Among Gram negative organisms, most common isolates were Salmonella typhi and Acinetobacter species(32%) followed by Escherichia coli (13.5%), Klebsiella species (8%),Pseudomonas species (8%) and Citrobacter species (5%).1.02% of Candida albicans was also isolated.Conclusion: The present study showed that most of the blood culture isolates, whether Gram positive or Gram negative were multidrug resistant. The rise in antibiotic resistance in blood culture isolates emphasizes the importance of rational and judicious use of antibiotics according to the antibiotic resistance pattern of that institution.
Keywords: Blood Culture, Antimicrobial Susceptibility, Gram Positive Cocci, Gram Negative Bacilli.
Ashina single, Sweta Gupta, Dinesh Jain, Pushpa Durlabhji. A STUDY OF BLOOD CULTURE ISOLATES AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN IN A TERTIARY CARE HOSPITAL OF JAIPUR, RAJASTHAN International Journal of Current Research and Review. 5(11), June, 56-63
1. Diekma DJ, Beekman SE, Chapin KC. Epidemiology and outcome of nosocomial and community on set blood stream infection. J Clin Microbiol 2003;41:3655-60.
2. Karlowsky JA, Jones ME, Draghi DC, Thornsberry C, Sahm DF, Volturo GA. Prevalence and antimicrobial susceptibilities of bacteria isolated from blood cultures of hospitalized patients in the United States in 2002. Annals of clinical microbiology and antimicrobials 2004;3:7.
3. Lorente C, Castillo YD,and Rello J.Prevention of infection in the intensive care unit: current advances and opportunities for the future. Current Opinion in Critical Care 2002, 8:461–464
4. Yagupsky P, Nolte FS. Quantitative aspects of septicaemia. Clin Microbiol Rev 1990;3:269-79.
5. Wisplinghoff H,Bischoff T, Tallent SM,Seifert H,Wenzel R P and Edmond M B. Nosocomial Bloodstream Infections in US Hospitals: Analysis of 24,179 Cases from a Prospective Nationwide Surveillance Study. Clinical Infectious Diseases 2004; 39:309– 17
6. Sharma M, God N, Chaudhary U, Aggarwal R, Arora DR. Bacteraemia in children. Indian J Pediatr 2002; 69: 1029 – 32
7. Graham C. Bacteremia and antibiotic resistance of its pathogens reported in England and wales between 1990 and 1998: Trend analysis. Br Med J 2000; 320: 213 – 216.
8. Clinical and Laboratory Standard Institute. Performance standards for antimicrobial disk susceptibility tests. NCCLS documents M 100 – SIS, 940 West Valley Road. Wayne, PA, USA, 2005; 19087.
9. Forbes BA. Bailey Scott’s diagnostic microbiology, 10th ed. St. Louis, Missouri, Mosby. 1998: 283–304.
10. Murty D S, Gyaneshwari M. Blood cultures in paediatric patients: A study of clinical impact. Indian J Med Microbiol 2007;25:220-4
11. Garg A., Anupurba S., Garg, J. and Sen, M. R. (2007). Bacteriological profile and antimicrobial resistance of blood culture isolates from a University hospital. Journal of the Indian Academy of Clinical Medicine 8(2), 139-143.
12. Arora U Devi P. Bacterial profile of blood stream infections and antibiotic resistance pattern of isolates. J K Sci 2007; 9:186-190.
13. Kavitha Prabhu, Sevitha Bhat, Sunil Rao Bacteriologic profile and antibiogram of blood culture isolates in a pediatric care unit Journal of laboratory Physicians Year : 2010, Volume : 2, Issue : 2, Page : 85-88.
14. Bichitrananda Swain and Sarita otta Bloodstream infections in a teaching hospital,Annals of Biological Research,2012,3(4):192 1928.
15. Meremikwu MM, Nwachukwu C E, Asuquo A E, Okebe J U and Utsalo S J. Bacterial isolates from blood cultures of children with suspected septicaemia in Calabar, Nigeria. BMC Infectious Diseases 2005, 5:110
16. Vanitha rani n, kannan gopal, venkata narendra m, vishwakanth d, v r d nagesh, yogitha m, venkata sunil m, thennarasu palani a retrospective study on blood stream infections and antibiotic susceptibility patterns in a tertiary care teaching hospital International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 4, Issue 1, 2012.
17. Karki S, Rai GK, Manandhar R. Bacteriological analysis and antibiotic sensitivity pattern of blood culture isolates in Kanti Children hospital. J Nepal Paediatric Soc 2010; 30(2):94-97.
18. Mehta MP. Dutta, V Gupta. Antimicrobial susceptibility pattern of blood isolates from a teaching hospital in North India. Jpn J Infect Dis 2005;58:174-176.
19. Anbumani N, Kalyani J, Mallika M. Original research distribution and antimicrobial susceptibility of bacteria isolated from blood cultures of hospitalized patients in a tertiary care hospital. Indian Journal for the practicing doctor 2008;5(2):2008-05 - 2008- 06
20. Barati M, Taher M T, Abasi R, Zadeh M M, Barati M, Shamshiri A R. Bacteriological profile and antimicrobial resistance of blood culture isolates. Iranian Journal of Clinical Infectious Diseases 2009;1(4):87-95.
21. Roy I, Jain A, Kumar M, Agarwal SK. Bacteriology of neonatal septicaemia in a tertiary care hospital of Northern India. Indian J Med Microbiol 2002;20 :156-9.