IJCRR - 5(11), June, 2013
Pages: 10-29
ANTIFUNGAL ANTIBIOTIC OF STREPTOMYCES SP. SS12: PRODUCTION AND CHARACTERIZATION
Author: Shipra Singh, Anita Rawat, Deepak Chand Sharma
Category: General Sciences
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Abstract:
Streptomyces sp. (strain SS12) was isolated from terrestrial soil of western U.P., India and found to exhibit antimicrobial activity against various pathogenic bacteria and fungi. Optimization of physical and chemical parameters for antibiotic production in submerged fermentation resulted in 1.75 fold increase in production level in terms of inhibition zone diameter. The bioactive compound was found thermo stable (121?C for 15min.), pH stable (from 4-9) and soluble in water and ethyl acetate. The antifungal antibiotic was found to have comparable activity with nystatin and could completely inhibit the spore germination and degradation of mycelia in test fungus (Aspergillus sp.). On supplementing 0.5% of the crude culture filtrate containing bioactive compound resulted in 76% inhibition of growth in submerged culture in compare to control.
Keywords: Antifungal antibiotic, Actinomycetes, Optimization, Streptomyces sp. SS12, Bioactive.
Citation:
Shipra Singh, Anita Rawat, Deepak Chand Sharma. ANTIFUNGAL ANTIBIOTIC OF STREPTOMYCES SP. SS12: PRODUCTION AND CHARACTERIZATION International Journal of Current Research and Review. 5(11), June, 10-29
References:
1. WHO 2012: Department of Communicable Disease Surveillance and Response: WHO Global Strategy for Containment of Antimicrobial Resistance. WHO/CDS/DRS/2001 1.2 [http://www.who.int/csr/resources/publicati ons/drugresist/en /EGlobal_Strat.pdf].
2. Xu SX, Shen JL, Tang XF, Feng B. Newer antifungal agents for fungal infection prevention during hematopoietic cell transplantation: a meta-analysis. Transplantation Proceedings 2013; 45(1): 407-414.
3. Berdy J. The discovery of new bioactive microbial metabolites: screening and identification. In: Bushell ME, Grafe U (eds) Bioactive metabolites from microorganisms, Elsevier Science Publications, Amsterdam. 1989. pp. 3-25.
4. Busti, E., P. Monciardini, L. Cavaletti, R. Bamonte, A. Lazzarini, M. Sosio, and S. Donadio. Antibiotic-producing ability by representatives of a newly discovered lineage of actinomycetes. Microbiol. 2006, 152:675-683.
5. Farid, M.A., el-Enshasy, H.A., el-Diwany, A.I. and el-Sayed el-S.A. Optimisation of the cultivation medium for natamycin production by Streptomyces natalensis J. Basic Microbiol, 2000. 40(3): 157-166.
6. Fiddaman, P.J. and Rossall, S. Effect of substrate on production of antifungal volatiles by Bacillus subtilis J. Appl. Bacteriol. , 1994. 76(4): 395-405.
7. Fukai T, Yonekawa M, Hou AJ, Nomura T, Sun HD, Uno J. Antifungal agents from the roots of Cudrania cochinchinensis against Candida, Crytococcus and Aspergillus species. J. Nat. Prod., 2003. 66: 1118-1120.
8. Gallis HA, Drew RH, Pickard WW. Amphotericin B: 30 years of clinic experience. Rev. Infect. Dis., 1990. 12: 308- 329.
9. Getha K, Vikineswary S. Antagonistic effects of Streptomyces violaceusniger strain G10 on Fusarium oxysporum f. sp. cubense race 4: Indirect evidence for the role of antibiosis in the antagonistic process. J. Ind. Microbiol., 2002. Biotechnol., 28: 303-10.
10. Hague, S.F., S.K. Sen and S.C. Pal. Nutrient optimization for production of broad spectrum antibiotic by streptomyces antibioticus SR 15. 4. Acta- Microbial. Immunol. Hune. 1995. 42 (2): 155-162.
11. Hassan MA, El-Naggar MY, Said WY. Physiological factors affecting the production of an antimicrobial substance by Streptomyces violatus in batch cultures. Egypt. J. Biol. 2001. 3: 1-10.
12. Mukherjee G, Sen SK. Purification, Characterization, and antifungal activity of chitinase from Streptomyces venezuelae P10. Curr Microbiol. 2006. 53: 265-9.
13. Mustafa Oskay. Antifungal and antibacterial compounds from Streptomyces strains. African Journal of Biotechnology, 2009. Vol. 8 (13), pp. 3007-3017. 14. Oskay M. Effects of some environmental conditions on biomass and antimicrobial metabolite production by Streptomyces sp., KGG32. Int J Agric Biol., 2011. 13:317-24.
15. Ouhdouch Y, Barakate M, Finance C. Actinomycetes of Moroccan habitats: Isolation and screening for antifungal activities. Eur. J. Soil Biol., 2001. 37: 69-74.
16. Prapagdee B, Kuekulvong C, Mongkolsuk S. Antifungal Potential of Extracellular Metabolites Produced by Streptomyces hygroscopicus against Phytopathogenic Fungi. Int. J. Biol. Sci., 2008. 4: 330-337.
17. Shiburaj, S. Screening, isolation and characterization of an antibiotic producing Actinomycete Streptomyces setonii 19NRA1 (Ph.D thesis), 2003. University of Kerala.
18. Singh S, Rawat A, and Sharma DC. 2012. Antifungal antibiotic production by Streptomyces sp. isolated from soil. International Journal of Current Research and Review, 4 (24), 07 -16.
19. Tarasova, S.S., V.V. Biryukov and V.G. Makarevich. Double-substrate equations of kinetics of Actinomyces aureofaciens growth and tetracycline biosynthesis. Antibiotiki. 1977. 22(44): 291-297.
20. Valois D, Fayad K, Barasubiye T, Garon M, Dery C, Brzezinski R, Beaulieu C. Glucanolytic actinomycetes antagonistic to Phytophthora fragariae var. rubi, the causal agent of raspberry root rot. Appl. Environ. Microbiol. 1996. 62: 1630-5.












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