A CURRENT REVIEW ON ASSESSMENT OF GENETIC ANALYSIS SYSTEM FOR MEDICINAL PLANTS WITH MICROSATELLITES

Nitin Kumar Verma; Ashwani Kumar; R. K. Deshwal; Vikas Tyagi; Priyanka Rana

Search Articles

Track manuscript

Abstract

IJCRR - 5(12), June, 2013

Pages: 01-11

A CURRENT REVIEW ON ASSESSMENT OF GENETIC ANALYSIS SYSTEM FOR MEDICINAL PLANTS WITH MICROSATELLITES

Author: Nitin Kumar Verma, Ashwani Kumar, R. K. Deshwal, Vikas Tyagi, Priyanka Rana

Category: General Sciences

[Download PDF]

Abstract:

Microsatellites or Simple Sequence Repeats (SSR) have been preferred molecular markers ever since their advent in the late eighties. SSR are tandem repeat units of 1 to 6 base pair that are found in many prokaryotic and eukaryotic genomes. Despite growing rivalry from new sequencing and genotyping techniques, the use of these mobile and efficient markers continues to increase, boosted by consecutive technical advances. Random determined expansions look to be elite against for at least part of microsatellite loci, probably because of their effect on organization of chromatin, gene regulation, recombination, replication of DNA, cell-division cycle, mismatch repair system, etc. The illustration methods used previously for finding new microsatellite loci in sand files remain difficult and time consuming; insilico approach, which includes retrieval and investigation of microsatellites from large amounts of sequence data from sequence data based using microsatellite tools can yield many new candidate markers. To make the most of the latest technical developments and outline the need for a well-established strategy including standardized high-throughput bench protocols and specific bioinformatics tools, from primer design to allele calling. In this review also cover the role of microsatellites in identification and isolation of genotypes, characterization and analysis of genetic diversity, and their interactions in SSR variation.

Keywords: Genetic Diversity, Molecular Marker, Simple Sequence Repeats, Phylogenetic Relationship

Citation:

Nitin Kumar Verma, Ashwani Kumar, R. K. Deshwal, Vikas Tyagi, Priyanka Rana. A CURRENT REVIEW ON ASSESSMENT OF GENETIC ANALYSIS SYSTEM FOR MEDICINAL PLANTS WITH MICROSATELLITES International Journal of Current Research and Review. 5(12), June, 01-11

References:

1. Alatar AA, Mahmoud MA, Al-Sohaibani SA, Abd-Elsalam KA.Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants.citatioGenet Mol Res. 2012 Feb 13; 11(1): 348-54.

2. Baldauf C, Ciampi MB, Vigna BB, Mori GM, Guedes JP, De Souza AP, Dos Santos FA.Characterization of microsatellite loci in Himatanthus drasticus (Apocynaceae), a medicinal plant from the Brazilian savanna.Am J Bot. 2011 Sep; 98(9): e244-6.

3. Deepa G, Venkatachalam L, Bhagyalakshmi N, Shashidhar HE, Singh V, Naidu KA Physicochemical and genetic analysis of an endemic rice variety, Njavara (Oryza sativa L.), in comparison to two popular South Indian cultivars, Jyothi (PTB 39) and IR 64 J Agric Food Chem. 2009 Dec 23; 57(24): 11476-83.

4. Deng KJ, Zhang Y, Xiong BQ, Peng JH, Zhang T, Zhao XN, Ren ZL. Identification, characterization and utilization of simple sequence repeat markers derived from Salvia miltiorrhiza expressed sequence tags. Yao Xue Xue Bao. 2009 Oct; 44(10): 1165-72.

5. Gao W, Zhao H, Liu Y, Li MR, Nurmamat E, Li LF, Ren YY, Xiao HX. Isolation and Identification of Fourtn Meeicrosatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae).Int J Mol Sci 2012; 13(8): 9609-14.

6. Guan Z, Zhang L, Song M, Li H, Zhang Z. Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker. Zhongguo Zhong Yao Za Zhi. 2012 Jun; 37(11): 1550-2.

7. Geistlinger J, Weising K, Winter P, Kahl G. Locus-specific microsatellite markers for the fungal chickpea pathogen Didymella rabiei (anamorph) Ascochyta rabiei. Mol Ecol. 2000 Nov; 9(11): 1939-41.

8. Haber LH, Cavallari MM, Santos FR, Marques MO, Gimenes MA, Zucchi MI. Development and characterization of microsatellite markers for Lychnophora pinaster: a study for the conservation of a native medicinal plant. Mol Ecol Resour. 2009 May; 9(3): 811-4.

9. Hadian J, Azizi A, Tabatabaei MF, Naghavi MR, Jamzad Z, Friedt W.Analysis of the genetic diversity and affinities of different Iranian Satureja species based on SAMPL markers.Planta Med. 2010 Nov; 76(16): 1927-33.

10. He J, Wang H, Li DZ,Chen SF Genetic diversity of Paris polyphylla var. yunnanensis, a traditional Chinese medicinal herb, detected by ISSR markers Janta Med. 2007 Oct; 73(12): 1316-21.

11. Harris ES, Klooster MR.Development of microsatellite markers for the medicinal plant Isodon rubescens (Lamiaceae) and related species.Am J Bot. 2011 Oct; 98(10): 293-5. 12. Hüttel B, Winter P, Weising K, Choumane W, Weigand F, Kahl G Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.). Genome. 1999 Apr; 42(2):210-7.

13. Hon CC, Chow YC, Zeng FY, Leung FC. Genetic authentication of ginseng and other traditional Chinese medicine. Acta Pharmacol Sin. 2003 Sep; 24(9): 841-6.

14. Hufford KM, Kochert G, Hamrick JL. Microsatellite primers and amplification of aborted embryos in Platypodium elegans J. Vogel (Fabaceae, Papilionoideae).Mol Ecol. 2000 Aug; 9(8):1174-6.

15. Heubl G.New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques.Planta Med. 2010 Dec; 76(17): 1963-74.

16. Joshi RK, Kar B, Nayaand Schisandra sphenanthera revealed by nuclear microsatellites.S21.Exploiting EST databases for the mining and characterization of short sequence repeat (SSR) markers in Catharanthus roseus L. Bioinformation. 2011 Feb 7; 5(9): 378-81.

17. Koblízková A, Dolezel J, Macas J. Subtraction with 3' modified oligonucleotides eliminates amplification artifacts in DNA libraries enriched for microsatellites. Biotechniques. 1998 Jul; 25(1): 32-4.

18. Kubaláková M, Lysák MA, Vrána J, Simkoá H, Cíhalíková J, Dolezel J. Rapid identification and determination of purity of flow-sorted plant chromosomes using CPRINS. Cytometry. 2000 Oct 1; 41(2): 102-8.

19. .Koblízková A, Dolezel J, Macas J. Chromosomal localization and distribution of simple sequence repeats and the Arabidopsistype telomere sequence in the genome of Cicer arietinum L Chromosome Res. 1998 Feb; 6(2): 97-104.

20. Koblízková A, Dolezel J, Macas J. Construction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L.).Chromosome Res. 1996 Nov; 4(7): 531-9.

21. Liao H, Zhao Y, Zhou Y, Wang Y, Wang X, Lu F, Song Z.Microsatellite markers in the traditional Chinese medicinal herb (Cucurbitaceae).Am J Bot. 2011 Mar; 98(3): e61-320.

22. Li Y, Sun C, Luo HM, Li XW, Niu YY, Chen SL. Transcriptome characterization for Salvia miltiorrhiza using 454 GS FLX. Yao Xue Xue Bao. 2010 Apr; 45(4): 524-9.

23. Liu H, Xia T, Zuo YJ, Chen ZJ, Zhou SL.Development and characterization of microsatellite markers for Panax notoginseng (Araliaceae), a Chinese traditional herb.Am J Bot. 2011 Aug; 98(8): e218-20.

24. Liu H, Xia T, Zuo YJ, Chen ZJ, Zhou SLDevelopment and characterization of microsatellite markers for Panax notoginseng (Araliaceae), a Chinese traditional herb.Am J Bot. 2011 Oct; 98(10): e274-6.

25. Liu X, Liu Z, Yang M, Chen KL. Study on diversity of Liriope spicata var. prolifera and its affinis species with ISSR method .Zhong Yao Cai. 2010 Jul; 33(7): 1052-5.

26. Luo H, Li Y, Sun C, Wu Q, Song J, Sun Y, Steinmetz A, Chen S.Comparison of 454- ESTs from Huperzia serrata and Phlegmariurus carinatus reveals putative genes involved in lycopodium alkaloid biosynthesis and developmental regulation.BMC Plant Biol. 2010 Sep 21; 10(5):10- 209.

27. Miao Y, Lang X, Li S, Su J, Wang Y. Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance. Int J Mol Sci 2012; 13(9): 11165-72.

28. Novy A, Jones KC Characterization of polymorphic microsatellites for Tripterygium (Celastraceae), a monospecific genus of medicinal importance.Am J Bot. 2011 Oct; 98(10): 280-1.

29. Rossetto M, Slade RW, Baverstock PR, Henry RJ, Lee LS. Microsatellite variation and assessment of genetic structure in tea tree (Melaleuca alternifolia-Myrtaceae). Mol Ecol. 1999 Apr; 8(4): 633-43.

30. Sarwat M, Das S, Srivastava PS.Estimation of genetic diversity and evaluation of relatedness through molecular markers among medicinally important trees: Terminalia arjuna, T. chebula .Mol Biol Rep. 2011 Nov; 38(8): 5025-36.

31. Shi HM, Wang J, Wang MY, Tu PF, Li XB. Identification of Cistanche species by chemical and inter-simple sequence repeat fingerprinting. Biol Pharm Bull. 2009 Jan; 32(1): 142-6.

32. Sarwat M, Das S, Srivastava PS. Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb Plant Cell Rep. 2008 Mar; 27(3): 519-28.

33. Shokeen B, Choudhary S, Sethy NK, Bhatia S.Development of SSR and gene-targeted markers for construction of a framework linkage map of Catharanthus roseus.Ann Bot. 2011 Aug; 108(2): 321-36.

34. Sorrell TC, Chen SC, Ruma P, Meyer W, Pfeiffer TJ, Ellis DH, Brownlee AG. Concordance of clinical and environmental isolates of Cryptococcus neoformans var. gattii by random amplification of polymorphic DNA analysis and PCR fingerprinting. J Clin Microbiol. 1996 May; 34(5): 1253-60.

35. Sorrell TC, Chen SC, Ruma P, Meyer W, Pfeiffer TJ, Ellis DH, Brownlee AG.Sorrell TC, Chen SC, Ruma P, Meyer W, Pfeiffer TJ, Ellis DH, Brownlee AG. The potential of microsatellites for hybridization- and polymerase chain reaction-based DNA fingerprinting of chickpea (Cicer arietinum L.) and related species. Electrophoresis. 1995 Sep; 16(9): 1755-61.

36. Sundaresan V, Sahni G, Verma RS, Padalia RC, Mehrotra S, Thul ST.Impact of geographic range on genetic and chemical diversity of Indian valerian (Valeriana jatamansi) from northwestern Himalaya Biochem Genet. 2012 Oct 5; 11 (79-10): 797- 808.

37. Tnah LH, Lee CT, Lee SL, Ng KK, Ng CH, Hwang SS.Microsatellite markers of an important medicinal plant, Eurycoma longifolia (Simaroubaceae), for DNA profiling.Am J Bot. 2011 May; 98(5): e130- 2.

38. Thul ST, Darokar MP, Shasany AK, Khanuja SP Molecular profiling for genetic variability in Capsicum species based on ISSR and RAPD markers.Mol Biotechnol. 2012 Jun; 51(2): 137-47.

39. Thul ST, Srivastava AK, Singh SC, Shanker KGenetic and chemical diversity of high mucilaginous plants of Sida complex by ISSR markers and chemical fingerprinting. Mol Biotechnol. 2011 Sep; 49(1): 77-81.

40. Udupa SM, Baum M. High mutation rate and mutational bias at (TAA)n microsatellite loci in chickpea (Cicer arietinum L.). Mol Genet Genomics. 2001 Aug; 265(6): 1097-103.

41. Udupa SM, Robertson LD, Weigand F, Baum M, Kahl G. Allelic variation at (TAA)n microsatellite loci in a world collection of chickpea (Cicer arietinum L.) germplasm. Mol Gen Genet. 1999 Mar; 261(2): 354-63.

42. Vardevanian PO, Minasbekian LA, Parsadanian MA DNA homology in various strains of nitrogen-fixing bacteria. Mol Gen Mikrobiol Virusol. 2000; (1): 27-30. Russian.

43. Winter P, Pfaff T, Udupa SM, Hüttel B, Sharma PC, Sahi S, Arreguin-Espinoza R, Weigand F, Muehlbauer FJ, Kahl G. Characterization and mapping of sequencetagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Mol Gen Genet. 1999 Aug; 262(1): 90-101.

44. Xie ML, Hou BW, Han L, Ma YH, Ding XY Development of microsatellites of Dendrobium officinale and its application in purity identification of germplasm. Yao Xue Xue Bao. 2010 May; 45(5): 667-72.

45. Y, Wen X, Huang H.Genetica..Genetic diversity and population structure of two important medicinal plant species Schisandra chinensis 2011 Apr; 139(4): 497-503.

46. Yu K, Park SJ, Poysa V, Gepts P. Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). J Hered. 2000 NovDec; 91(6): 429-34.

47. Yan Y, Wang Z, Tian W, Dong Z, Spencer DF.Generation and analysis of expressed sequence tags from the medicinal plant Salvia miltiorrhiza. Sci China Life Sci.2010 Feb; 53(2): 273-85.

48. Yuan YH, Hou BW, Xu HJ, Luo J, Ding XYIdentification of the geographic origin of Dendrobium thyrsiflorum on Chinese herbal medicine market using trinucleotide microsatellite markers Biol Pharm Bull. 2011; 34(12): 1794-800.

49. Zanella CM, Bruxel M, Paggi GM, Goetze M, Buttow MV, Cidade FW, Bered Genetic structure and phenotypic variation in wild populations of the medicinal tetraploid species Bromelia antiacantha (Bromeliaceae).Am J Bot. 2011 Sep; 98(9): 1511-9.

50. Zhan QQ, Sui C, Wei JH, Fan SC, Zhang J. Construction of genetic linkage map of Bupleurum chinense DC. using ISSR and SSR markers.Yao Xue Xue Bao. 2010 Apr; 45(4): 517-23.

51. Zheng JY, Wang H, Chen XX, Wang P, Gao P, Li XNMicrosatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae.,.Genet Mol Res. 2012 Aug 6; 11(3): 1975-80.

52. Zhang ZR, Yang J, Sun Y, Gao TG, Li DZ, Xue CY.A set of novel microsatellite markers developed for the traditional Tibetan medicinal plant Halenia elliptica (Gentianaceae).Am J Bot. 2011 Jul; 98(7): e173-5.

53. Zeng S, Xiao G, Guo J, Fei Z, Xu Y, Roe BA, Wang Y. Development of a EST dataset and characterization of EST-SSRs in a traditional Chinese medicinal plant, Epimediumsagittatum (Sieb. Et Zucc.) Maxim. BMC Genomics. 2010 Feb 8; 11: 94

. 54. Zhou YQ, Jing JZ, Li ZY, Zhang BH, Jia JF. Assessment of genetic diversity of Rehmannia glutinosa germplasm detected by RAPDs and ISSRs. Yi Chuan. 2004 Nov; 26(6): 922-8.

Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

COPE guidelines for Reviewers

SCOPUS indexing: 2014, 2019 to 2021

Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award:

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Best Article Award

A study by Dorothy Ebere Adimora et al. entitled \"Remediation for Effects of Domestic Violence on Psychological well-being, Depression and Suicide among Women During COVID-19 Pandemic: A Cross-cultural Study of Nigeria and Spain\" is awarded Best Article of Vol 14 issue 23

A study by Muhas C. et al. entitled \"Study on Knowledge & Awareness About Pharmacovigilance Among Pharmacists in South India\" is awarded Best article for Vol 14 issue 22

A study by Saurabh Suvidha entitled \"A Case of Mucoid Degeneration of Uterine Fibroid with Hydrosalphinx and Ovarian Cyst\" is awarded Best article of Vol 14 issue 21

A study by Alice Alice entitled \"Strengthening of Human Milk Banking across South Asian Countries: A Next Step Forward\" is awarded Best article of Vol 14 issue 20

A study by Sathyanarayanan AR et al. entitled \"The on-task Attention of Individuals with Autism Spectrum Disorder-An Eye Tracker Study Using Auticare\" is awarded Best article of Vol 14 issue 19

A study by Gupta P. et al. entitled \"A Short Review on \"A Novel Approach in Fast Dissolving Film & their Evaluation Studies\" is awarded Best Article of Vol 14 issue 18.

A study by Shafaque M. et al. entitled \"A Case-Control Study Performed in Karachi on Inflammatory Markers by Ciprofloxacin and CoAmoxicillin in Patients with Chronic Suppurative Otitis Media\" is awarded Best Article of Vol 14 issue 17

A study by Ali Nawaz et al. entitled \"A Comparative Study of Tubeless versus Standard Percutaneous Nephrolithotomy (PCNL) \? A Randomized Controlled Study\" is awarded Best Article for Vol 14 issue 16.

A study by Singh R. et al. entitled \"A Prospective Study to Find the Association of Astigmatism in Patients of Vernal Keratoconjunctivitis (VKC) in a Tertiary Health Care Centre in India (Vindhya Region MP)\" is awarded Best Article for Vol 14 issue 15

A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14

A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13

A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12

A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11

A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10

A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09

A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08

A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07

A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06

A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05

A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04

A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03

A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02

A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01

A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24

A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23

A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22

A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21

A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20

A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19

A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18

A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17

A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16

A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15

A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14

A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13

A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12

A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11

A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10

A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09

A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08

A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07

A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness

A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health

A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06

A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia

A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05

A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04

A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03

A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02

A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01

A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24

A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23

A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22

A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21

A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20

A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19

A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18

A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17

A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16

A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15

A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020

A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"

A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13

A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for Vol 12 issue 12

A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for Vol 12 issue 11

A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for Vol 12 issue 10

A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for Vol 12 issue 09

A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for Vol 12 issue 08

A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for Vol 12 issue 07

A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20

A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR

A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13

A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR

A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR.

A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award

A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award

RSS feed

IJCRR

A CURRENT REVIEW ON ASSESSMENT OF GENETIC ANALYSIS SYSTEM FOR MEDICINAL PLANTS WITH MICROSATELLITES

RSS feed

ABOUT US

Useful links

Useful links

Contact