IJCRR - vol 05 issue 13, July, 2013
INCIDENCE OF HIV INFECTION AMONG KNOWN PULMONARY TUBERCULOSIS PATIENTS AND PULMONARY TUBERCULOSIS AMONG KNOWN HIV SEROPOSITIVE INDIVIDUALS; AND A COMPARATIVE STUDY OF ZIEHL-NEELSEN STAINING AND AURAMINE-O STAINING
Author: S. Saraswathi, T.V. Ramani, Supriya Panda
To know HIV seropositivity among known pulmonary tuberculosis individuals and sputum smear positivity for acid fast bacilli among known HIV seropositive individuals and to do a comparative study of Ziehl-Neelsen staining and Auramine-O staining. Methods: 500 symptomatic patients attending to Designated Microscopic Centre were screened for pulmonary tuberculosis by doing Ziehl-Neelsen staining of sputum samples. Sputum smear positive individuals were screened for HIV antibodies. 500 individuals attending to Integrated Counseling and Testing Centre were screened for HIV antibodies according to National AIDS Control Organization guidelines. HIV seropositive individuals were screened for pulmonary tuberculosis by doing Ziehl-Neelsen stain of sputum smear. Auramine O staining was done for randomly selected sputum samples. Results: Incidence of pulmonary tuberculosis was 15.2 %, and among them 5.3 % were HIV seropositive. Incidence of HIV infection was 16.2 %, out of them two were having pulmonary tuberculosis. Out of 50 sputum smear samples selected randomly, 32 were positive for acid fast bacilli in Ziehl-Nelseen stain, 34 were positive by Auramine O stain. Conclusion: In the present study incidence of HIV infection among pulmonary tuberculosis patients was 5.2% and pulmonary tuberculosis among HIV infected individuals was 2.5%. Auramine O stain for detecting M. tuberculosis was more sensitive than Z-N stain.
Keywords: HIV- TB Co-infection, Auramine-O staining
S. Saraswathi, T.V. Ramani, Supriya Panda. INCIDENCE OF HIV INFECTION AMONG KNOWN PULMONARY TUBERCULOSIS PATIENTS AND PULMONARY TUBERCULOSIS AMONG KNOWN HIV SEROPOSITIVE INDIVIDUALS; AND A COMPARATIVE STUDY OF ZIEHL-NEELSEN STAINING AND AURAMINE-O STAINING International Journal of Current Research and Review. vol 05 issue 13, July, 99-104
1. TB India. RNTCP, 2012, Annual Status Report.
2. Sharma SK, Saha PK, Dixit Y, Siddaramaiah NH, Seth P,Pande JN. HIV Seropositivity among adult tuberculosis patients in Delhi. Indian J Chest Dis Allied Sci 2000; 42: 157- 60.
3. Solomon S, Anuradha S, Rajasekaran S. Trend of HIVinfection in patients with pulmonary tuberculosis in south India. Tuber Lung Dis 1995; 76: 17-9.
4. Paranjape RS, Tripathy SP, Menon PA, Mehendale SM, Khatavkar P, Joshi DR, et al. Increasing trend of HIV seroprevalence among pulmonary tuberculosis patients in Pune, India. Indian J Med Res 1997; 106: 207-11.
5. Samuel NM, Alamelu C, Jagannath K, Rajan B. Detection of HIV infection in pulmonary tuberculosis patients. J Indian Med Assoc 1996; 94: 331-3.
6. Sharma SK, Aggarwal G, Seth P, Saha PK. Increasing HIV seropositivity among adult tuberculosis patients in Delhi. Indian J Med Res 2003; 117: 239-42.
7. Mohanty KC, Nair S, Sahasrabudhe T. Changing trend ofHIV infection in patients with respiratory disease inBombay since 1988. Indian J Tuberc 1994; 41: 147-50.
8. Mohanty KC, Basheer PMM. Changing trend of HIV infection and tuberculosis in a Bombay area since 1988.Indian J Tuberc 1995; 42: 117-20.
9. Purohit SD, Gupta RC, Bhattara VK. Pulmonarytuberculosis and Human Immunodeficiency Virus infection in Ajmer. Lung India 1996; 14: 113-20.
10. Banvaliker JN, Gupta R, Sharma DC, Goel MK, KumariS. HIV seropositivity in hospitalized pulmonary tuberculosis patients in Delhi. Indian J Tuberc 1999; 44:17-20.
11. Gupta PR, Luhadia SK, Gupta SN, Joshi V. Tuberculosis in human immunodeficiency virus seropositives inRajasthan. Indian J Tuberc 1998; 16:147-9.
12. Talib SH, Bansal MP, Kamble MM. HIV-1 seropositivity in pulmonary tuberculosis (study of 340 cases from Marathwada). Indian J Pathol Microbiol 1993; 36: 383-8
13. Dr. V. Sivaraman and Dr. Gilbert Fernandez, TB Sanatorum, Gorimedu, Pondicherry, Dr. R. Sambasiva Rao AIDS Surveillance Centre, JIPMER, Pondicherry. Indian J Tuberculosis.1992; 39:35-39.
14. S.K. Sharma, P.K. Saha, Yogita Dixit, Naveen H. Siddaramaiah, Pradeep Sethand J.N. Pande Departments of Medicine and Microbiology, All India Institute of Medical Sciences, New Delhi, HIV Seropositivity Among Adult Tuberculosis Patients in Delhi. India Indian J Chest Dis Allied Sci 2000; 42: 157-160.
15. Deswal B.S, D. Bhatnagar, D. Kumar, VR Deshpande, A Study of Prevelance of HIV in Tuburculosis Cases. Indian Journal of Community Medicine. Vol. 27, No. 2 (2002-04 - 2002-06)
16. Piramanayagam.P, Mohammad Tahir, S.K. Sharma, Duncan Smith-rohrberg, A. Biswas and M. Vajpayee. Persistently high HIV seroprevalence among adult tuberculosis patients at a tertiary care Centre in Delhi. Indian J Med Res. February 2007; 125 163- 166.
17. Singh N P, Parija S C. The value of fluorescent microscopy of auramine stained sputum smears for diagnosis of pulmonary Tuberculosis.Southeast Asian J Med Public Health 1998 Dec; 29 (4): 860.
18. Prasanthi. K , Kumari A R, Efficacy of flurochrome staining in the diagnosis of pulmonary tuberculosis Co infected with HIV. Indian Journal of Medical Microbiology. 2005.Vol 23,No 3,179-185
19. Kumar N, Tiwari M C, Verma K AFB staining in cytodiagnosis of Tuberculosis without classical features: A comparison of Zeihl- Neelsen and fluorescent methods.Cytopathology,1998 May;vol 9 (3):208 -214.