IJCRR - Vol 09 Issue 05, March, 2017
Effect of Sprouting Time on Antioxidant Properties of Trigonella foenum-graecum (Fenugreek) Seeds Available in Delhi-NCR Region
Author: Sadhna Jain1, Punita Saxena2, Heena Lamba3
Aim: Fenugreek seeds are popular for their diverse therapeutic benefits. Processing intervention to improve nutritional properties of such agricultural seeds is an age old practice. In the current study, sprouted fenugreek seeds have been analyzed for their potential use as a home based approach for the management of oxidative stress related disorders.
Methodology: Germinated seeds were dried at 60°C, powdered and stored at 4°C. The samples extract (10 mg/mL) in distilled water were then analyzed for Total Phenolic Content (TPC) and antioxidant activity by DPPH and FRAP. The results obtained for seven days were compared for significant differences using one-way ANOVA.
Results: The process of sprouting showed significant changes in the antioxidant properties of fenugreek seeds with the maximum activity observed on 7th day of sprouting. An increase ranging from 40% to 45% in TPC and antioxidant activity was observed on the seventh day of germination. A correlation analysis among the TPC and antioxidant activity (DPPH and FRAP) was also performed that confirmed a high value of R2 (0.965, 0.956) between the two.
Conclusions: Fenugreek seeds at seventh day of sprouting have the prospect to be used as nutraceutical for the management of oxidative stress related disorders.
Keywords: Total phenolic content, DPPH, FRAP, Antioxidant activity, Fenugreek seeds
Sadhna Jain1, Punita Saxena2, Heena Lamba3. Effect of Sprouting Time on Antioxidant Properties of Trigonella foenum-graecum (Fenugreek) Seeds Available in Delhi-NCR Region International Journal of Current Research and Review. Vol 09 Issue 05, March, 33-37
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