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IJCRR - 6(23), December, 2014

Pages: 68-74

Date of Publication: 10-Dec-2014


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THE EFFECTS OF GIBBERELLIC ACID ON QUALITY AND SHELF LIFE OF BANANA (MUSA SPP.)

Author: Tolossa Duguma, Meseret C. Egigu, Manikandan Muthuswamy

Category: General Sciences

Abstract:Banana (Musa spp.) is one of the most important fruit crops of the tropical and subtropical areas over the world. Worldwide, postharvest losses in fruits and vegetables range from 25 to 40% and this high loss is due to lack of packaging, storage facilities andpoor means of transportation. So, this experiment aimed at evaluating the efficiency of postharvest treatments to improve the storage of banana fruits by using gibberellic acid (GA3). Mature green banana fruits were treated by immersion in 100, 200 and 300ppm of GA3 for 15 minutes. The control fruits were immersed in distilled water the same way. Then, all fruits were stored in the lab at about 13\?2\?C temperature for 35 days and evaluated for different quality parameters over six periods (0, 7, 14, 21, 28 and 35 days). All fruits treated with GA3 delayed in ripening in concentration dependent manner when compared to control fruits. The ripening of fruits treated with 300ppm GA3 delayed more than other treatments. The treatment with higher concentration of gibberellic acid delayed peel color changes, weight loss, ethylene, CO2 production and total sugar content as compared with control groups. In the meanwhile, the pulp to peel ration and total soluble solids were increased with increasing the concentration of gibberellic acid treatments. This indicate that gibberellic acid prevent the fruit ripening. Therefore, postharvest application of gibberellic acid was an efficient method to delay banana fruit ripening. As gibberellic acid concentration increased, ripening was further delayed.

Keywords: Postharvest loss, Gibberellic acid 3, Ripening, Musa spp.

Full Text:

INTRODUCTION
Banana is cultivated in more than 120 countries over about 10 million hectares. It ranks first in global fruit production with just over 106 million tons being produced annually worldwide. Currently, banana is most exported fruit and ranks second after citrus fruits in terms of value over the world. Total world exports of banana in 2006 accounted for 16.8 million tons (FAOSTAT, 2006). In Ethiopia, banana is one of the widely produced and used tropical fruits. From introduced and locally collected varieties, Dwarf Cavendish, Giant Cavendish, Poyo and Ducasse hybrid were recommended for production in Ethiopia (Seifu, 1999). The area under cultivation and the total annual production of banana in the year 2000 were 15,000 ha and 100,000 tones, respectively. This grew to 39,428 ha and 260,000 tones, respectively in 2008. Most of the banana produced in Ethiopia are consumed locally with only a few exported to Djibouti, Saudi Arabia and Somalia (FAO, 2010. IJCRR Section: General Science Banana is a fragile, perishable fruit and cannot be preserved for longer time after harvesting (Taiwo and Adeyemi, 2009). It is highly desirable to delay or postpone the ripening and senescence until they are to be consumed (Ramana et al., 1989).Therefore, the current study is aimed at extending the shelf life and keeping quality of banana fruits by using different concentrations of gibberellic acid 3 (100, 200 and 300ppm).

MATERIALS AND METHODS
Banana fruits of Giant Cavendish cultivar were obtained from Melkassa Research center, Ethiopia. From the collected banana fruits, approximately equal sized fruits with no bruises or damage were selected for treatments with different concentrations of gibberellic acid GA3 (100, 200 and 300ppm) separately. The experimental design was a complete randomized design with three replications. For each GA3 treatments, 10 banana fruits were randomly assigned to prepared concentrations and immersed into their respective concentrations for 15 minutes. After 15 minutes of immersion, fruits were made to dry in air for 30 minutes. The control group was only washed with distilled water and air dried in the same way and then all fruits packed into labeled carton box, and stored for 35 days at temperature of 13 + 2 o C in the lab. Thereafter, fruit quality assessments with respect to the following parameters were carried out 6 times in7 day’s interval.

FRUIT COLOR DETERMINATION
Fruit peel color analysis was assessed visually by matching the peel color with standardized color charts that describe the seven ripening stages, and color score were assigned accordingly;1 = full green, 2 = green with yellow tip, 3 = greener than yellow, 4 = more yellow than green, 5 = yellow with green tip, 6 = fully yellow, 7 = yellow with black spots. The banana was considered unripe at stages 1-4, and ripe at stages 5-7 (Li et al., 1997).

Determination of Pulp to Peel Ratio and Physiological weight loss
The ratios of pulp to peel of each finger was calculated and mean value were recorded (Dadzie and Orchad, 1997). The physiological weight loss was determined by using method indicated by Teferra et al. (2007) Ethylene and CO2 Determination Ethylene production was measured as described by Jiang et al. (2004). Banana fruit was placed in a sealed 1.5 L jar at 20°C and incubated for 1hr to accumulate ethylene. 10ml of gas was then taken from the headspace of the jar using a gastight syringe, and injected into a gas chromatograph (Type CG, 9002 German) equipped with a flame ionization detector to determine the identity of ethylene and quantify it. For CO2 analysis, 1mL of gas was taken from the headspace of the jar using a gastight syringe and was injected into a gas chromatograph equipped with a flame ionization detector to determine its identity and quantity. Known concentrations of ethylene and CO2 standards both in synthetic air were used to generate calibration curves so as to estimate their concentrations.

Measurement of Vitamin C (Ascorbic acid) Content and Titratable Acidity (TA)
Ascorbic Acid (AA) content of banana was determined by the 2, 6-dichlorophenolindophenol method (AOAC, 2000). Measurement of titratable acidity was calculated as percentage of malic acid since it is the dominant acid in banana (Dadzie and Orchad, 1997).

Measurement of Total Soluble Solid and Total sugar content
Total soluble solids (TSS) were determined following the procedures described by Wasker et al. (1999). Total sugars were estimated by using the techniques of Seyoun (2002).

Statistical Analysis
Data obtained were subjected to analysis of variance and mean comparison using the SAS computer software version 9.1. Pearson’s correlation test was used to determine the correlation between each parameter. The p-value less than 0.05 were considered statistically as significant.

RESULTS AND DISCUSSION
Peel Color Change The peel color showed the significant difference between treatments on each sampling day (P<0.001)(Fig.1). The fastest color change was observed in control (untreated fruits) where fruits reached fully yellow color (stage 7) on the 14th day. On the same day, fruits treated with gibberellic acid 3 (GA3)) were at advanced peel color development. Fruits treated with 300 and 200ppm GA3 were at stage 2 (highly delayed in color change) and fruits treated with 100ppm were at stage 4. On day 21st all fruits treated under control group was fully ripe and showed yellow color with increasing brown spot after which all fruits in this group were discarded. On the same day fruits treated with 300, 200 and 100ppm were at stage 3rd, 4th and 5th, respectively. In the same way, on 35th day of storage fruits treated with 100ppm GA3 were at stage 7 (yellow color with increasing brown color) while fruits treated with 300 and 200ppm were at stage 6 (fully yellow). The observed delay in color change of banana fruits treated with GA3 as compared to banana fruits in control group could be due to the retarding effect of this hormone on the synthesis of ethylene and, hence, reduced the respiration rate of the fruits in concentration dependent manner. The peel color development from green to yellow in this study is in agreement with the work of (Dharmasenal and Kumari, 2005; Salvador et al., 2006). The results are also in agreement with previous reports that GA3 retarded color development in banana (Ahmed and Tingwa., 1995).

Pulp to Peel Ratio
Effect of GA3 acid on pulp to peel ratio of banana fruit have been investigated during ripening and showed significant difference (P ≤ 0.001) except on 0th day during storage period. Starting from day 7 th all treatments showed significant differences throughout storage period (Fig.2). On day 7th storage period, the observed pulp to peel ratio for control fruit was 2.15 while that of fruits treated with GA3 was below 1.84. In the same way on day 14th, the observed pulp to peel ratio for fruits treated with GA3 was below 2 while that of control fruit was 2.66. On 21st day, the pulp to peel ratio of banana fruit treated under control group was 3.14 (which was over ripe and after which all of fruits were discarded) while that of fruits treated with 100, 200 and 300ppm GA3 were 2.45, 2.32 and 2.23, respectively. On 35th day the pulp to peel ratios of fruits treated with 100 and 200ppm GA3 were 2.76 and 2.68, respectively while the minimum ratio (2.62) was observed for fruits treated with 300ppm GA3. There was an increase in the pulp to peel ratio of banana fruit during the storage period for all fruits, but banana fruits treated with GA3 showed slight increase in pulp to peel ratios. Pulp to peel ratio suggested a delay in ripening of banana fruit in the presence of GA3. The increase in pulp to peel ratio is due to the displacement of water from the peel towards the fruit pulp during the ripening process, resulted from the osmotic pressure gradient from the higher sugar concentration of the pulp relative to the peel. The averages obtained in the experiment were between 1.43 and 2.82, similar to the numbers reported by Cerqueira et al. (2000).

Physiological Weight Loss (%)
Variation was observed in the percent weight loss of banana fruits treated with different concentrations of GA3 except on 0th day. The differences were significant (P ≤ 0.001) among all treatments during the storage period of 35th days (Fig.3). The highest percentage of weight loss was recorded for control fruits on each sampling day up to 21st days. On day 21st, the percentage of weight loss for control fruit was 19.86% after which all fruits in this group were discarded. But, banana fruits treated with GA3 showed reduced weight loss significantly compared to control. There were also significant differences between all treatments on day 28th. The maximum %weight loss was recorded for fruits treated with 100 and 200ppm (12.89 and 12.17%), respectively while the minimum was recorded for 300ppm (11.68%). There were also significant differences (P ≤ 0.001) for each treatment on 35th day of storage. The minimum %weight loss were observed in fruits treated with 300ppm GA3 (15.15) which shows delay in ripening and maximum percent weight loss were recorded for fruits treated with 100ppm GA3 (16.99%) while values recorded for fruits treated with 200ppm GA3 was (16.63%). The result is in line with the work of Dharmasenal and Kumari, (2005) that Excess energy produced from the respiration process in the form of heat is released from the fruit by evaporation of water causing a weight loss. Similarly, increase in the membrane permeability following the respiratory climacteric could result in loss of moisture through the peel (Siriboon and Banlusilp, 2004). Ethylene production The effects of the different concentrations of GA3 showed significant difference (P ≤ 0.001) on Ethylene production throughout the storage period. Banana fruits treated with GA3 showed minimum ethylene production while control group showed maximum production of ethylene. On the first day of storage, fruits treated under control group produced maximum amount of ethylene (11.70μl/ kg/hr) while those treated with 300, 200 and 100ppm GA3 produced lower amount (9.42 ,9.54 and 9.86μl/kg/ hr) respectively. On 7th day of storage, ethylene production increased in all treatments but highly increased in control groups (18.55μl/kg/hr) while slight increase in GA3 treated fruits (all are below 11μl/kg/hr). The ethylene production increased in all treatments and rose to 62.12μl/kg/hr in control group on 28th day and declined to 49.20μl/kg/hr on 35th day fruit treated with GA3 (Fig.4) The result was in line with the work of Seymour et al. (1993) who reported that unripe bananas show a constant low level of ethylene production. At the onset or ripening, ethylene production rose up, which was followed by a high rate of respiration. The pre-climacteric stage started from the first day to the 21st day, while the climacteric rose on the 21st day to the 28th day, and reached a climacteric peak on the 28th day, then, entered its senescence on the 35th day and onwards.

Effect on Respiration Rate (CO2 production)
The effects of the different concentrations of GA3 showed significant difference (P ≤ 0.001) on CO2 production during storage period (Fig. 5). Control fruits produced highest amount of CO2 during storage period while banana fruits treated with GA3 showed lower amount of CO2 production in concentration dependent manner. On the first day of storage control fruits produced 27.4ml/kg/hr while those treated with 300 and 200ppm GA3 produced 24.6 and 24.8ml/kg/hr, respectively. On the 7th day of storage carbon dioxide production increased in all treatments, but highly increased in untreated fruits (49.8mL/ kg/hr) while slight increase in GA3 treated fruits. Fruits treated with 300, 200 and 100ppm GA3 produced 36, 37 and 38.5ml/kg/h, respectively. The production of CO2 increased in all treatments and rose to 116.0ml/kg/hr in control group on 28th day and declined to 79.7ml/kg/hr on 35th day while GA3 treated fruits still showed gradual increase in CO2 production and each treatment produced less than 80 ml/kg/hr.

Generally, in control fruits the rate of respiration was found to increase with ripening exhibiting a peak on day 28th (climacteric peak). GA3 treatment resulted in a lower rate of respiration as well as a delay in appearance of climacteric peak in a concentration dependent manner, as compared to control. There was a gradual increase in respiration rate of GA3 treated fruits up to 35th day of storage period, suggesting a delay in ripening. As observed from the current study 300ppm GA3 is the concentrations of choice in delaying banana fruit ripening by inhibiting CO2 production (respiration rate). This agrees with previous reports that GA3 delays the onset of the climacteric peak in banana fruits (Ahmed and Tingwa 1995). Ascorbic Acid A significant (P≤0.001) difference was observed in the ascorbic acid (AA) content of banana fruits receiving different postharvest treatments except on 0th day (Fig.6). Control fruits showed a rapid decrease in AA content than fruits treated with GA3. On 21st day of storage the AA content of banana fruit placed in control group decreased from 34.81 to 15.18 mg/100gfw while fruits treated with 300 and 200ppm GA3 reduced to 26.51 and 25.14mg/100gfw, respectively. In similar way, on 35th day of storage period the AA content of banana fruits treated with 100, 200 and 300ppm were reduced to14.30, 15.81 and 16.53mg/100gfw, respectively. There was a progressive decrease in ascorbic acid content up to the end of shelf life. The decrease in ascorbic acid on prolonged storage might be mainly due to rapid conversion of L-ascorbic acid into dehydro ascorbic acid in the presence of enzyme ascorbinase. The difference in the AA levels among fruits in the different treatments could be due to the effects of respective treatments on ripening as described for other parameters in the earlier sections. These results show that concentration of 300ppm GA3 is the most effective in delaying ripening of banana fruits. The result of the current study is in agreement with the report of Wenkam (1990) and Lee and Kader (2000) who indicated a decline in AA content of banana fruits during ripening. It is also in line with the work of Ghanta (1994) who reported that ascorbic acid content declined from 33.30mg/100gfw to 10.64 mg/100gfw during ripening.

Titratable Acidity (%)
Treatment of different concentrations of GA3 showed significant difference (P ≤ 0.001) on TA (titratable acidity) content of banana fruits throughout the storage period except on day 0th (Fig.7). On 7th day of storage period, control fruits reached its peak (0.66%) and thereafter started to decline and reached 0.36% on 21st day of storage period after which all fruits in this group were discarded. The TA content of fruits treated with 300, 200 and 100ppm GA3 reached their peaks (0.58, 0.62 and 0.59% respectively) on21stday of storage period and thereafter started to decline up to the end of storage period (35th day).This result is in agreement with observations by Siriboon and Banlusilp (2004) who reported that TA increased to its peak, which coincided with the accumulation of ethylene and ripening, then started to decline afterwards. Hernandez et al. (2006) also reported the amount of TA at; unripe, half ripe and ripe maturity stages were 0.30, 0.57 and 0.45 g /100gfw, respectively. This range is similar to the present observation, but with a slight difference, which could be due to cultivar difference. Total Soluble Solids Postharvest treatments with different concentrations of GA3 are significantly (P ≤ 0.001) affected the TSS (total soluble soilds) content of banana fruits (Fig.8). Control fruits showed a faster increment in their TSS content, increasing from 2.29 ºBrix on day 0th to 22.90 ºBrix on day 14th and declined to 16.82 ºBrix on day 21st, after which all fruits in this group were discarded. On the other hand, fruits treated with GA3 were the slowest to reach their TSS peak. Banana fruits treated with 100 and 200ppm GA3 reached their peak (22.85 and 22.34ºBrix, respectively) on day 28th while fruits treated with 300ppmGA3 were still increasing in its TSS and reached its peak (22.00ºBrix) on day 35. The observed increment in TSS content during ripening of fruits and decrease after attaining peak levels followed natural fruit ripening and senescence processes that have also been exhibited in related traits including color change and fruit marketability which are typical of postharvest change in climacteric fruit (Pinto et al., 2004). This result is in agreement with the report of Dharmasenal and Kumari (2005) that showed increase in TSS contents of different banana varieties from 0 to 17th °Brix over a storage period of 16 days. The slightly more amount of TSS at the peak of ripening in the present study could be due to differences in the type of cultivars studied (Dadzie and Orchard, 1997) and effects of the treatments applied.

Total Sugar Contents
Postharvest treatments with different concentrations of GA3 were significantly (P ≤ 0.001) affected the total sugar content of banana fruits (Fig.9). Banana fruits treated with different concentrations of GA3 showed slight increase in total sugar contents during storage period and decreased after reaching their peaks in concentration dependent manner while untreated fruits showed rapid increase to their peak and decrease thereafter. On day 0th the total sugar content of control fruit was 0.84 g /100gf.w and reached its peak 20.98g /100gfw on 14th day of storage period and thereafter started to decline. On 21st day of storage period total sugar content of all fruits treated under control group were reduced to 16.89g /100gfw after which all fruits in this group were discarded. But, total sugar content of banana fruits treated with GA3 were still increasing and reached their peak on 28th day and started to decline on 35th day of storage period except fruits treated with 300ppm GA3, which was still increasing up to 35th day of storage period. In the current study, the sugar content of the fruits during the storage period ranged from 0.85g/ 100gfw to 21.45 g /100gf.w which are in agreement with that reported by Robinson (1996). The author reported that the fruits declined from starch content of about 20.0 to 23.0 g /100gfw at harvest to 1.0 to 2.0 g/ 100gfw in ripe fruits, while the amount of sugar increased in about the same proportion. Rapid increment in the total and reducing sugar as well as a rapid decrease in sugar contents of control fruit could be due to faster ripening process which converts starch in to sugar, while the slower rate in the rest of the treatments could be due to the effects of the treatments in delaying the ripening process (Golding et al., 2005).



CONCLUSIONS
Postharvest application of gibberellic acid was an efficient method of delaying banana fruit ripening. Fruit treated with gibberellic acid had dramatically delayed the fruits peel color changes, weight loss, ethylene, co2 production and total sugar content. As gibberellic acid content increased, ripening was further delayed as compared with control groups. Thus, with the concentrations tested, gibberellic acid allows growers to schedule banana fruit ripening.

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A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

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