International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
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IJCRR - 4(8), April, 2012

Pages: 85-88

Date of Publication: 25-Apr-2012


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PHYTOCHEMICALS ANALYSIS OF COLECUS AROMETICUS BENTH

Author: Iffat Khan, Kirti Jain

Category: General Sciences

Abstract:Coleus aromaticus Benth. Of the family Lamiaceae is a large succulent aromatic herb used for flavoring drinks and medicine. The leaves are considered as carminative, digestive, anthelmintic and diuretic. The present study is dealt with the phytochemicals analysis of the leaf extracts. The leaf extracts with various solvents like petroleum ether extract, Ethanolic extract and Aqueous extract were prepared and both qualitative and quantitative tests for phytochemicals were done.It showed the presence of saponins,carbohydrates, tannins,flavonoids,proteins,triterpenoids in various quantities.

Keywords: Colecus, Ethanolic , saponins, phytochemicals.

Full Text:

INTRODUCATION
About two and a half species of flowering plants belonging to 10,500 genera and about 300 families are found in nature .of these a number of genera are source of drugs. Many of the plant products are important therapeutic agents, which are represented by the various phytochemicals like saponins,carbohydrates, tannins,flavonoids, proteins, triterpenoids. The present study is concentrated on colecus arometicus Benth., belongs to family Lamiaceae.Although phytochemicals are not classified as nutrients, substances necessary for sustaining life, they have been identified as containing properties for aiding in disease prevention.phytochemical are associated with the prevention and treatment of at least four of the leading causes of death in the united statescancer,Diabetes,cardiovascular disease, and hypertensation. They are involved in many processes including one that helps prevent cell damage, prevent cancer cell replication, and decrease cholesterol levels.

MATERIALS AND METHODS
Suitable explants will be taken from the authenticated and identified plant taxonomist. The identified and authenticated species were collected quantity, dried and powdered for further studies. The phytochemicals present in the plant material was extracted by the distillation method using soxhlet apparatus. Different solvent, solvent systems were used for the sepration of chemicals according to the polarity (petroleum ether extract, Ethanolic extract, aqueous extract) about 650 g of plant tissue were weighed and shade dried for 10 days. The dried materials were powdered and 50g of powder sample was packed in a thimble and kept in soxhlet apparatus. Each of the solvent was taken separately for the extraction and the powdered material was siphoned by 3 times. The whole apparatus was kept over a heating mantle and was heated continuously for 8 hours at boiling ponit of each solvent. The extract was concentrated to dryness and the residues were transferred to a pre-weighed sample bottle and were stored in desiccators for further studies.

"Phytochemicals screening of different plant extracts found by soxhlat method. in different media"
Different biochemical parameters like, saponins,carbohydrates, tannins,flavonoids,proteins,triterpenoids. 1. Test for flvonoids =Take 1 ml of the extract and add few magnesium turnings, followed by the addition of conc.HCL drop. Development of pink colour indicates the presence of flavonoids is present by ethanolic extract and aqueous extract. 2. Terpenoids = Take 2mlof extract, dry and dissolve in chloroform. Add a few drops of acetic anhydride and conc.H2S04 and keep undisturbed for few minutes. Formation of pink colour indicates the presence of terpenids by petroleum ether extract. 3. Test for tannins and phenolic compound = To 1ml of the extract, add 2 drops of 5% Fecl3. Presence of dirty green precipitate indicates the presence of tannins and phenolic compound by ethanolic extract and aqueous extract. 4. Test for saponins =5ml of the extract was shaken with 5ml of distilled water and was heated to the boiling point. Froathing indicates the presence of saponins by ethanolic extract and aqueous extract. 5. Total carbohydrate=weighed amount of fresh tissue was homogenized with distilled water. The homogenate was filtered using a two layered cheese cloth. The filtrate was then centrifuged at 10,000g for 15 min. The supernatant was collected and the volume was made up to 25 ml using distilled water .an aliquot of sample was pippetted out and 4ml anthrone reagent added. It was then kept in a boiling water bath for 10 min. The tubes were cooled and the absorbance was measured at 530nm. The amount of total carbohydrate present was determined using the standard graph of glucose.

 

tannins,flavonoids,triterpenoids in the soxhlet method . Study showed that the extract of plant contain most phytochemicals. Such as ?‘ saponins,carbohydrates, tannins,flavonoids,proteins,triterpenoids‘‘

 

DISCUSSION
Phytochemical compound of the plant were analyzed. In the analysis of phytochemical compound of colecus arometicus Benth extract showed the presence of ?‘saponins,carbohydrates, tannins,flavonoids,proteins,triterpenoids‘‘ arometicus used to treat asthma for a long time with any undesirable side effect . colecus arometicus is responsible for its pharmaceutical value.

CONCLUSION
Phytochemical analysis showed that the presence of terpenoids and phenolic compounds were responsible for the effects. Phytochemicals are also found in colecus arometicus, hence it may be the reason for the activity. In short, based on the present study it was abserved that the plant extracts, colecus arometicus in various phytochemicals like ‘saponins,carbohydrates, tannins,flavonoids,proteins,triterpenoids‘‘ In view of the above, present study was undertaken keeping mainly following objects:-

  • Study of phytochemicals of colecus arometicus Benth
  • Secondary metabolite analysis of colecus arometicus Benth.

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Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

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A study by Muhas C. et al. entitled \"Study on Knowledge & Awareness About Pharmacovigilance Among Pharmacists in South India\" is awarded Best article for Vol 14 issue 22
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A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
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A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
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A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
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A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
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A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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