International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
logo
slider
slider
slider
slider
Bootstrap Slider

Indexed and Abstracted in: Crossref, CAS Abstracts, Publons, Google Scholar, Open J-Gate, ROAD, Indian Citation Index (ICI), ResearchGATE, Ulrich's Periodicals Directory, WorldCat (World's largest network of library content and services)

Search Articles

Track manuscript

Full Html

IJCRR - 4(19), October, 2012

Pages: 62-68

Date of Publication: 15-Oct-2012


Print Article   Download XML  Download PDF

GENETIC DIVERSITY IN FIVE CICER GENOTYPES USING OPM-05 PRIMER

Author: Jahangir Ahmad, Vaseem Raja, Rajdeep Kudesia

Category: General Sciences

Abstract:Five genotypes of Cicer (Dhawal, Ujjwal, Shubhra, DCP-92-3 and IPC-04-20) were analyzed to estimate genetic diversity by using OPM-05 primer. A total of 21 bands were detected, all of them were polymorphic. Similarity index calculated was found highest between Ujjwal and IPC-04-20 (37.5%) and lowest between Shubhra and IPC-04-20 (22.22%). Dice's similarity coefficient ranged from 0.00000 to 0.25000. A dendrogram constructed based on UPGMA revealed two clusters. Cluster 1 consists of three accessions-S1, S3 and S5 while as cluster 2 consists of two accessions- S2 and S4. It is evident from the dendrogram that accession DCP-92-3 and IPC-04-20 are genetically dissimilar; hence it is recommended that these should be used for future breeding programs to create higher amount of genetic variability in chickpea.

Keywords: Genetic diversity, Cicer, RAPD, UPGMA dendrogram

Full Text:

INTRODUCTION
The genus Cicer includes 33 perennials, eight annuals and one unspecified wild species as well as the cultivated chickpea (Van der Maesen 1987). Chickpea is the second most important cool season pulse crop in the world and is grown in atleast 33 countries including central and west Asia, South Europe, Ethiopia, North Africa, North and South America and Australia (Ladizinsky and Adler 1976; Singh and Ocampo 1997). Chickpea is the most important pulse crop in India. It is commonly grown in Uttar Pradesh, Panjab, Maharashtra, Rajasthan, Bihar and Madhya Pradesh. These together accounts for more than 90% of the total area under it. There are two broad groups of gram-brown and white. The brown or desi type is most widely grown. The white or kabuli is characterized by larger seeds. In India, gram is sown as Rabi crop at the end of the rainy season. Sowing takes place from September to November, and harvesting from February to April. India is the largest producer of chickpea, accounting for 66% of the world production (FAO 2004). The average annual yield world wide (0.78 ton/ hectare) is considered to be somewhat lower than its potential yield (Singh et al., 1994; Sudupak et al., 2002). Assessment of the extent of genetic variability within chickpea is fundamental for chickpea breeding and the conservation of genetic resources and is particularly useful as a general guide in the choice of parents for breeding hybrids. Criteria for the estimation of genetic diversity can be different which include morphological traits (Upadhaya et al., 2007) or molecular markers (Sharma et al., 1995). Molecular markers have proved to be useful tools in the characterization and evaluation of genetic diversity within and between species and populations. A no. of DNA based markers is now available for the effective quantification of genetic variation in plant species. Restriction fragment length polymorphism (RFPL) and Amplified fragment length polymorphism (AFLP) have been applied successfully and have provided considerable genetic information in a no. of plant species (Vos et al., 1995, Xu et al., .2000). These techniques are slow and expensive and are not amenable for assessment of genetic variation in large scale population genetic studies. More recently PCR based RAPD and simple sequence repeat (SSR) markers requiring small amounts of DNA have also been developed (Williams et al., 1990). SSR markers have been proved to be polymorphic but require nucleotide information for primer design (Sun et al., 1998). RAPD methodology overcomes this limitation; considerable polymorphic markers can be obtained with relative ease from minute amounts of genomic DNA without prior knowledge of sequence information RAPD is a PCR based technique first developed by Williams et al., (1990) widely used in molecular biology. Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplification products of anonymous DNA sequences using single, short and arbitrary oligonucleotide primers, and thus don’t require prior knowledge of a DNA sequence. Low expense, efficiency in developing a large number of DNA markers in a short time and requirement for less sophisticated equipment has made the RAPD technique valuable although reproducibility of the RAPD profile is still the centre of debate (Bardakcl F 2001).

MATERIALS AND METHODS
The germplasm were obtained from Indian Institute of Pulse Research, Kanpur (U.P.) India. During the present study, five accessions of Cicer were used to estimate genetic diversity by using RAPD analysis. The details of five accessions are given in table 1.

RAPD analysis For RAPD, 1g seed of each accession was used to isolate the genomic DNA Genomic DNA isolation The problem of DNA extraction is still an important issue in the field of plant molecular biology. Various plants contain high levels of polysaccharides and many types of secondary metabolites affecting DNA purification (Zidani et al., 2005). CTAB procedure based on the protocol of Doyle and Doyle (1990) is the method of choice for obtaining good quality DNA from many plant species and also from fungi (Weising et al., 1991). CTAB is a cationic detergent which solubilizes membranes and forms a complex with DNA. One gram seed of each accession was ground to a fine powder in liquid nitrogen then transferred to PCR tubes and an equal volume of hot (650C) 2X CTAB was added to it. The mixture was incubated at 650C for 10 minutes and mixed it with an equal volume of chloroform/isoamyl alcohol (24: 1) and centrifuged at 13000 g for 5 minutes. The aqueous phase was then transferred to another tube and 1/10 volume of 10 X CTAB was added to it, mixed and treated with an equal volume of chloroform/isoamyl alcohol. An equal volume of CTAB precipitation buffer was added to the aqueous phase to precipitate the DNA. The DNA pallet was rehydrated in high salt TE buffer and the DNA reprecipitated with 2 volumes of chilled ethanol. Finally the DNA pallet was air dried rehydrated in 0.1 X TE buffer and treated with RNase. Selection of the primer The decamer primer OPM-05 (5'GGGAACGTGT 3') used for RAPD analysis in chickpea was selected on the basis of prior study. It showed high polymorphism in the earlier studies also as revealed by Talebi et el 2009 and other workers. Polymerase Chain reaction PCR stand for polymerase chain reaction is a technique used to selectively amplify in-vitro a specific segment of the total genomic DNA a billion fold. The most essential requirement of PCR is the availability of a pair of short oligonucleotide called primers has sequences complementary to either end of the target DNA segment to be synthesized in large amounts (Ahmad et al., 2010). Amplification are carried out in a themocycler for about 35-40 cycles with an initial strand separation at 940C, 1 minute at 370C and 2 min. at 720C. Amplification products were electrophoresed in 2% agarose gels and detected by staining with ethiduim bromide. After electrophoresis, the RAPD patterns were visualized with UV transilluminator. RAPD markers were scored as DNA fragments present or absent. The bands scored were used for the construction of dendrogram by using UPGMA (Unweighted Pair Group Mean and Arithmetic Average) method to know the phylogenetic relationships among the five accessions of Cicer. RESULTS AND DISCUSSION The RAPD analysis of five Cicer genotypes was carried out to investigate genetic diversity using OPM-05 primer.RAPD markers represent an efficient and inexpensive way to generate molecular data, thus have been used successfully in various taxonomic and phylogenetic studies (Aboelwafa et al., 1995; Sharma et al., 1995; Friesen et al., 1997., Wolff and Morgan-Richards 1998). Only clearly scorable bands were included in the analysis. Minor bands which could not be scored reliably were not included in the analysis. In total 21 visible bands were scored in all accessions corresponding to an average of 4.2 bands per accessions. All bands were polymorphic (Table 2, Figure 1). Similar results were observed by Ahmad F (1999) in chickpea by using 75 random decamer primers. Several other workers also observed high polymorphism by using different random primers in different species. Sudupak et al., (2002) used seven decamer primers in chickpea and observed 96.82% polymorphism. Sharma et al., (2000) also recorded 100% polymorphism in Podophylum haxandrum by using seven decamer primers and SDS-PAGE. Jaya prakash et al., (2006) recorded 97.4% polymorphism in sorghum genotypes by using 64 RAPD primers. Aboelwafa et al., (1995) used 40 primers and recorded 90% polymorphism in Lens.Verma et al., (2009) used 20 random primers and observed 96.8% polymorphism in Trichodesma indicum. Iqbal et al., (2010) worked on Jatropa curcas L. and recorded 93.90% polymorphism by using 50 random primers. Chickpea genotype Dhawal produced the maximum number of bands (9), while Ujjwal gave the minimum number (4) of bands (Table 2, Figure 1).



The dendrogram obtained from the UPGMA method is shown in Figure 2. Cluster analysis divided the five accessions into two clusters. Cluster I comprising three accessions (Dhawal, Shubhra and IPC-04-20) while as the cluster II consists of two accessions (Ujjwal and DCP-92-3). It is evident from the dendrogram that Dhawal and Shubhra and Ujjwal and DCP-92-3 are very close to each other while as IPC-04-20 and DCP-92-3 are genetically dissimilar. Closely related species commonly lose the ability to interbreed and become genetically isolated due to chromosomal structural mutations (Tayyar and Wainess 1996). IPC-04-20 and DCP-92-3 are genetically distant, hence it is recommended that these two accessions should be used in future breeding programs to create higher amount of genetic variability in chickpea.

CONCLUSION
RAPD analysis is efficient in revealing usable level of DNA polymorphism among cultivars of chickpea. By this study, we can show how much diversity and similarity level is present in five cultivars of chickpea. We can also be able to identify genetic makeup of the variety to protect the breeder’s right. We can estimate the genetic distance among the cultivars of chickpea and calculate the similarity coefficient among the cultivars. It is suggested on the basis of RAPD analysis that chickpea cultivars DCP-92-3 and IPC-04-20 will produce higher hybrid vigour when they are utilized in breeding programs because they are genetically distinct from other cultivars of chickpea and have minimum genetic similarity. ACKNOWLEDGEMENT The authors are thankful to IIPR (Indian Institute of Pulse Research) Kanpur, India for providing germplasm. The lab facility provided by Dr. S. Chauhan and Mr. Sanjay are also greatly acknowledged.

References:

1. Aboelwafa, A., Murai, K. and Shimada, T. (1995).Intra and interspecific variations in Lens revealed by RAPD markers.Theoretical and Applied Genetics. 90:335-340.

2. Ahmad, F. (1999). Random amplified polymorphic DNA analysis reveals genetic relationships among the annual cicer species. Theoretical and Applied Genetics. 98:657- 663.

3. Ahmad, F., Khan, A.I., Awan, F.S.,Sadaqat, H.A.,Sadia, B. and Bhadur, S. (2010).Genetic diversity of chickpea (Cicerarietinum L.) germplasm in Pakistan as revealed by RAPD analysis. Genetic and Molecular Research.9 (3):1414-1420.

4. Bardakcl, F. (2001).Random Amplified Polymorphic DNA (RAPD) markers.Turk J Biol.25:185-196.

5. Doyle, J.J. and Doyle, J.J. (1990). Isolation of plant DNA from fresh tissue.Focus 12:13-15.

6. FAO. (2004).http//faostat.fao.org.

7. Friesen, N., Fritsch, R. and Bachmann, K. (1997). Hybrid origin of some ornamentals of Allium sub genus melanocrommyum verified with GISH and RAPD. Theoretical and Applied Genetic .95:1229-1238.

8. Ikbal, Boora, K.S.and Dilloon, R.S. (2010). Evaluation of genetic diversity in Jatropa curcas L. using RAPD markers. Indian Journal of Biotechnology. 9:50-57.

9. Jeyaprakash, S.P., Biji, K.R., Gomez, S.M., Murthy, K.G. and Babu, R.C. (2006).Genetic diversity analysis of sorghum [Sorghum bicolor (L.) Moench] accessions using RAPD markers. Indian Journal of Crop Sciences. 1(1-2):109-112.

10. Ladizinsky,G., and Adler,A.(1976).Genetic relationship among the annual species of Cicer L.Theor.Appl.Genet.48:197-203.

11. Sharma, K.D., Singh, B.M., Sharma, T.R., Katoch, M. and Guleria, S. (2000).Molecular analysis of variability in Podophylum hexandrum Royle- an endangered medicinal herb of north western Himalaya.Plant Genetic Resources Newsletter. 124:57-61.

12. Sharma, P.C., Winter, P., Bunger, T., Huttel, B., Weigand, F., Weising, K.and Kahl, G. (1995). Abundance and polymorphism of di, tri and tetra nucleotide tendem repeats in chickpea (Cicer arietinum L.). Theoretical and Applied Genetics. 90:90-6.

13. Singh, K.B., Malhotra, R.S.,Halila, H., Knights, E.J.andVerma, M.M.(1994).Current status and future strategy in breeding chickpea for resistance to biotic and abiotic stresses.Euphytica.73:137-149.

14. Singh, K.B.and Ocampo, B.(1997). Exploitation of wild species for yield improvement in chickpea.Theoretical and Applied Genetics. 95:418-423.

15. Sudupak, M.A., Akkaya, M.S. and Kence, A. (2002).Analysis of genetic relationships among perennial and annual Cicer species growing in Turkey using RAPD markers.Theoretical and Applied Genetics.108(5):937-944.

16. Sun, G.L., Salomon, B.and Bothmer, R.V. (1998). Charaterization and analysis of micro satellite loci in Elymus caninus (Triticea: Poaceae). Theoretical and Applied Genetics. 96:676-82.

17. Talebi, R., Jelodar, N.A.B., Mardi, M., Fayaz., Furman, B.J.andBagheri, N.A. (2009). Phylogenetic diversity and relationship among annual Cicer species using RAPD markers.General and Applied Plant Physiology. 35:3-12

18. Tayyar, R. I. and Waines, J. G. (1996).Genetic relationships among annual species of Cicer using isozyme variation.Theoretical and Applied Genetics. 92: 245-254.

19. Upadhaya, H.D., Dwividi, S.L., Gowds, C.L.L.and Singh, S. (2007). Identification of diverse germplasm lines for agronomic traits in chickpea (Cicer arietinum L.) core collection for use in crop improvement. Field Crops Res. 100:320-6.

20. Van der Maeswn, L.J.G.(1987). Origin, history and taxonomy of chickpea .In: Saxena, M.C., Singh, K.B., The chickpea.CAB Int Publ, UK: 11-34.

21. Verma, N., Koche, V., Tiwari, K.L. and Mishra, S.K. (2009). RAPD analysis reveals genetic variation in different populations of Trichodesma indicum-A perennial medicinal herb. African Journal of Biotechnology. 8(18):4333-4338.

22. Vos, P., Hogers, R., Blecker, M., Reijans, M., Lee, T.V.D., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zebeau, M., (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acid Res. 18:6531-5.

23. Weising, K., Ramash, J., Kaemmer, D., Kahl, G. and Epplen, J.T. (1991).Oligonucleotide fingerprinting in plants and fungi. pp. 312- 329. In: Burke, T., Dolf, G., Jeffery, A.J. and Wolff,R. (eds.) . DNA fingerprinting: Approaches and Applications, pp.312-390. BirkhauserVerlag, Basel, Switzerland.

24. Williams, J.G.K., Kubelic, A.R., Lival, K.J., Rafalski, J.A. and Tingy, S.V. (1990). DNA polymorphism amplified by arbitrary primers is useful as genetic markers. Nucleic Acids Res. 18:6531-6535.

25. Wolff, K. and Morgan Richards, M. (1998). PCR markers distinguish plantago major sub species. Theoretical and Applied Genetics. 96:282-286.

26. Xu, X., Peng, M., Fang, Z. and Xu, XP. (2000). The direction of microsatellite mutations is dependent upon allele length. Nature Genet.24:396-9.

27. Zidani, S., Ferchichi, A. and Chaieb, M. (2005). Genomic DNA extraction method from pearl millet (Pennisetum glaucum) leaves. African Jour

Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

COPE guidelines for Reviewers

SCOPUS indexing: 2014, 2019 to 2021


Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A study by Dorothy Ebere Adimora et al. entitled \"Remediation for Effects of Domestic Violence on Psychological well-being, Depression and Suicide among Women During COVID-19 Pandemic: A Cross-cultural Study of Nigeria and Spain\" is awarded Best Article of Vol 14 issue 23
A study by Muhas C. et al. entitled \"Study on Knowledge & Awareness About Pharmacovigilance Among Pharmacists in South India\" is awarded Best article for Vol 14 issue 22
A study by Saurabh Suvidha entitled \"A Case of Mucoid Degeneration of Uterine Fibroid with Hydrosalphinx and Ovarian Cyst\" is awarded Best article of Vol 14 issue 21
A study by Alice Alice entitled \"Strengthening of Human Milk Banking across South Asian Countries: A Next Step Forward\" is awarded Best article of Vol 14 issue 20
A study by Sathyanarayanan AR et al. entitled \"The on-task Attention of Individuals with Autism Spectrum Disorder-An Eye Tracker Study Using Auticare\" is awarded Best article of Vol 14 issue 19
A study by Gupta P. et al. entitled \"A Short Review on \"A Novel Approach in Fast Dissolving Film & their Evaluation Studies\" is awarded Best Article of Vol 14 issue 18.
A study by Shafaque M. et al. entitled \"A Case-Control Study Performed in Karachi on Inflammatory Markers by Ciprofloxacin and CoAmoxicillin in Patients with Chronic Suppurative Otitis Media\" is awarded Best Article of Vol 14 issue 17
A study by Ali Nawaz et al. entitled \"A Comparative Study of Tubeless versus Standard Percutaneous Nephrolithotomy (PCNL) \? A Randomized Controlled Study\" is awarded Best Article for Vol 14 issue 16.
A study by Singh R. et al. entitled \"A Prospective Study to Find the Association of Astigmatism in Patients of Vernal Keratoconjunctivitis (VKC) in a Tertiary Health Care Centre in India (Vindhya Region MP)\" is awarded Best Article for Vol 14 issue 15
A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


RSS feed

Indexed and Abstracted in


Antiplagiarism Policy: IJCRR strongly condemn and discourage practice of plagiarism. All received manuscripts have to pass through "Plagiarism Detection Software" test before Toto Macau forwarding for peer review. We consider "Plagiarism is a crime"

IJCRR Code of Conduct: To achieve a high standard of publication, we adopt Good Publishing Practices (updated in 2022) which are inspired by guidelines provided by Committee on Publication Ethics (COPE), Open Access Scholarly Publishers Association (OASPA) and International Committee of Medical Journal Editors (ICMJE)

Disclaimer: International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.



ABOUT US

International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

Contact

148, IMSR Building, Ayurvedic Layout,
        Near NIT Complex, Sakkardara,
        Nagpur-24, Maharashtra State, India

editor@ijcrr.com

editor.ijcrr@gmail.com


Copyright © 2024 IJCRR. Specialized online journals by ubijournal .Website by Ubitech solutions